1. Introduction -- 2. Laboratory Organization -- 3. Sterilization Techniques -- 4. Nutrition Medium -- 5. Choice of the Explant -- 6. Seed Culture -- 7. Micropropagation - Meristem Culture -- 8. Micropropagation - Axillary Bud Proliferation -- 9. Micropropagation - Adventitious Regeneration -- 10. Micropropagation - Organogenesis -- 11. Micropropagation - Embryogenesis -- 12. Cell Suspension -- 13. Secondary Metabolite Production in a Cell Suspension Culture -- 14. Anther Culture -- 15. Protoplast Isolation and Fusion -- 16. SDS-PAGE Electrophoresis of Proteins -- 17. Isolation of DNA from Plant Tissues -- 18. Isolation and Purification of Plasmid DNA -- 19. Restriction Enzyme Digestion of DNA -- 20. Agarose Gel Electrophoresis -- 21. Preparation of Competent Cells, Transformation of E.coli with Plasmid DNA and Ligation of Insert DNA to a Vector -- 22. Agrobacterium-mediated Gene Transfer -- 23. Biolistic Method of Transformation in Plants -- 24. In vitro Amplification of DNA by PCR: Detection of Transgenes -- 25. RAPD Analysis -- 26. Microsatellite Marker Analysis -- 27. Southern Blotting -- 28. Southern Hybridization -- App.I. Metric prefixes -- App.II. Greek alphabets -- App.III. Molarity concentrations of some commonly, used plant growth regulators -- App.IV. Composition of some plant tissue culture media -- App.V. Mineral and salt composition of Vacin and Went (1949) medium for Orchid culture -- App.VI. Mineral composition of some media commonly used in anther/microspore culture -- App.VII. 8p media for protoplast culture (Kao and Michyaluk, 1975) -- App.VIII. Protoplast Culture Medium PC I (Frearson et al., 1973) -- App.IX. K3 medium for protoplast culture (Nagy and Maliga, 1976) --App.X. Protoplast culture medium (Nagata and Takebe, 1971) -- App.XI. White's minerals used for protoplast culture (White, P.R., 1963) -- App.XII. Nucleic acid conversion factors -- App.XIII. Agarose gel percentages for resolution of linear DNA -- App.XIV. Polyacrylamide gel percentages for resolution of DNA -- App.XV. Trouble shooting guide during electrophoresis -- App.XVI. SOB medium composition -- App.XVII. Phenol equilibration, RNase prepar -- App.XVIII. YEM medium constitution -- App.XIX. Dendrogram construction

1. Plant biotechnology