The bacterial strain; SR-1, was isolated from infected leather, and identified as Bacillus licheniformis. This strain was found the capability to produce an antifungal and antibacterial antibiotics. The cultivated conditions for antibiotic production of SR-1 was optimized in shake flask scale. It was found that SR-1 was produced high amount of antibiotic in modified R-1 medium which consisted of 0.5% Bacto peptone, 0.3% Beef extract, 1.0% Maltose, 0.25% Yeast extract and 0.5x10-5 M. MnCl₂, initial pH 7.0, temperature 35°C on shaking flask at 200 rpm. The cells were harvested after cultivation for 60 hours. Under these conditions, 24.36 unit/mg. cell mass of antibiotic was obtained. The antibiotic from SR-1 was determined as an intracellular substances. The cells were broken by freeze-thawed followed by lysozyme treatment. Whole cells extract were concentrated in 50% glycerol and then partially purified twice through the SEP-PAK C₁₈ and Sephadex G-25, respectively. According to these system, antibiotic from strain SR-1 was 16-folds increase in purification. The purified antibiotic was inhibited both gram-poitive bacteria; Bacillus cereus ATCC 11778, Bacillus subtilis ATCC 6633, Bacillus megaterium ATCC 14581, Staphylococcus aureus ATCC 25923 and some fungi; Gliocladium sp. Paecilomyces variotii. Purified antibiotic was detected by RP-HPLC which showed single peak at retention time 2.15 minute. This antibiotic was estimated molecular weight about 9700 dalton, by using SDS-PAGE. To determine preliminary structure of purified antibiotic by the Infrared Spectrum, IR spectra appeared adsorption band at 3450, 1650 and 1450 cm, respectively. These indicated that antibiotic from strain SR-1 was peptide antibiotic. Purified antibiotic was mostly stable at emperature 30°C and pH 7.0-7.5; resisted to B-glucosidase, B-lactamase, lysozyme, papain and proteinase K, but slightly hydrolysed by pepsin and completely hydrolysed by trypsin.
