Title | Clinical Proteomics [electronic resource] : Methods and Protocols / edited by Antonia Vlahou |
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Imprint | Totowa, NJ : Humana Press, 2008 |
Connect to | http://dx.doi.org/10.1007/978-1-59745-117-8 |
Descript | XVI, 408 p. 74 illus. online resource |
Preface -- Acknowledgments -- Contributors -- 1. Overview and Introduction to Clinical Proteomics Young-Ki Paik, Hoguen Kim, Eun-Young Lee, Min-Seok Kwon, and Sang Yun Cho -- I: Specimen Collection for Clinical Proteomics -- 2. Specimen Collection and Handling: Standardization of Blood Sample CollectionHarald Tammen -- 3. Tissue Sample Collection for Proteomics Analysis Jose I. Diaz, Lisa H. Cazares, and O. John Semmes -- II: Clinical Proteomics by 2DE and Direct MALDI/SELDI MS Profiling -- 4. Protein Profiling of Human Plasma Samples by Two-Dimensional Electrophoresis Sang Yun Cho, Eun-Young Lee, Hye-Young Kim, Min-Jung Kang, Hyoung-Joo Lee, Hoguen Kim, and Young-Ki Paik -- 5. Analysis of Laser Capture Microdissected Cells by 2-Dimensional Gel Electrophoresis Daohai Zhang and Evelyn Siew-Chuan Koay -- 6. Optimizing the Difference Gel Electrophoresis (DIGE) Technology David B. Friedman and Kathryn S. Lilley -- 7. MALDI/SELDI Protein Profiling of Serum for the Identification of Cancer Biomarkers Lisa H. Cazares, Jose I. Diaz, Rick R. Drake, and O. John Semmes -- 8. Urine Sample Preparation and Protein Profiling by Two-Dimensional Electrophoresis and Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectroscopy Panagiotis G. Zerefos and Antonia Vlahou -- 9. Combining Laser Capture Microdissection and Proteomics Techniques Dana Mustafa, Johan M. Kros, and Theo Luider -- III: Clinical Proteomics by LC-MS Approaches -- 10. Comparison of Protein Expression by Isotope-Coded Affinity Tag Labeling Zhen Xiao and Timothy D. Veenstra -- 11. Analysis of Microdissected Cells by Two-Dimensional LC-MS Approaches Chen Li, Yi-Hong, Ye-Xiong Tan, Jian-Hua Ai, Hu Zhou, Su-Jun Li, Lei Zhang, Qi-Chang Xia, Jia-Rui Wu, Hong-Yang Wang, and Rong Zeng -- 12. Label-Free LC-MS Method for the Identification of Biomarkers Richard E. Higgs, Michael D. Knierman, Valentina Gelfanova, Jon P. Butler, and John E. Hale -- 13. Analysis of the Extracellular Matrix and Secreted Vesicle Proteomes by Mass Spectrometry Zhen Xiao, Thomas P. Conrads, George R. Beck, Jr., and Timothy D. Veenstra -- IV: Clinical Proteomics and Antibody Arrays -- 14. Miniaturized Parallelized Sandwich Immunoassays Hsin-Yun Hsu, Silke Wittemann, and Thomas O. Joos -- 15. Dissecting Cancer Serum Protein Profiles Using Antibody Arrays Marta Sanchez-Carbayo -- V: Statistics and Bioinformatics in Clinical Proteomics Data Analysis -- 16. 2D-PAGE Maps Analysis Emilio Marengo, Elisa Robotti, and Marco Bobba -- 17. Finding the Significant Markers: Statistical Analysis of Proteomic DataSebastien Christian Carpentier, Bart Panis, Rony Swennen, and Jeroen Lammertyn -- 18. Web-Based Tools for Protein Classification Costas D. Paliakasis, Ioannis Michalopoulos, and Sophia Kossida -- 19. Open-Source Platform for the Analysis of Liquid Chromatography-Mass Spectrometry (LC-MS) Data Matthew Fitzgibbon, Wendy Law, Damon May, Andrea Detter, and Martin McIntosh -- 20. Pattern Recognition Approaches for Classifying Proteomic Mass Spectra of Biofluids Ray L. Somorjai