Title | Antimicrobial Peptides [electronic resource] : Methods and Protocols / edited by Paul R. Hansen |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2017 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-6737-7 |
Descript | XIV, 439 p. 87 illus., 49 illus. in color. online resource |
Antimicrobial Peptides: An Introduction -- Tools for designing amphipathic helical antimicrobial peptides -- Chemical Synthesis of Antimicrobial Peptides -- Microwave-Assisted Synthesis of Antimicrobial Peptides -- High Performance Liquid Chromatography and Mass Spectrometry-Based Design of Protolytically Stable Antimicrobial Peptides -- Determination of Structure and Micellar Interactions of Small Antimicrobial Peptides by Solution-State NMR -- Mass spectrometry approaches for determining the structure of antimicrobial peptides -- Molecular Dynamics Simulation and Analysis of the Antimicrobial Peptides-Lipid Bilayers Interactions -- Calorimetry methods to study membrane interactions and perturbations induced by antimicrobial host defense peptides -- Fluorescence and absorbance spectroscopy methods to study membrane perturbations by antimicrobial host defense peptides -- Applying Fluorescence Correlation Spectroscopy to Investigate Peptide-Induced Membrane Disruption -- Macromolecule Biosynthesis Assay and Fluorescence Spectroscopy Methods to Explore Antimicrobial Peptide Mode(s) of Action -- Using Confocal Microscopy and Computational Modeling to Investigate the Cell Penetrating Properties of Antimicrobial Peptides -- Atomic Force Microscopy Study of the Interactions of Indolicidin with Model Membranes and DNA -- Protocols for studying the interaction of MSI-78 with the Membranes of Whole Gram-positive and Gram-negative Bacteria by NMR -- Preparation of membrane models of Gram-negative bacteria and their interaction with Antimicrobial Peptides studied by CD and NMR -- Studying the Interaction of Magainin 2 and Cecropin A with E. coli Bacterial Cells using Circular Dichroism -- Studying the Mechanism of Membrane Permeabilization induced by Antimicrobial Peptides using Patch-clamp Techniques -- Assays for Identifying Inducers of the Antimicrobial Peptide LL-37 -- Methods for Elucidating the Mechanism of Action of Proline-rich and other Non-lytic Antimicrobial Peptides -- The Interaction of Antimicrobial Peptides with the Membrane and Intracellular Targets of Staphylococcus Aureus Investigated by ATP-leakage, DNA-binding Analysis and the Expression of a LexA Controlled Gene, recA -- Methods for Investigating Biofilm Inhibition and Degradation by Antimicrobial Peptides -- Protocols for Studying Inhibition and Eradication of Bacterial Biofilms by Antimicrobial Peptides -- Protocols for Studying Antimicrobial Peptides (AMPs) as Anti-cancer Agents -- Using Oral and Colon Cancer Cells for studying the Anti-cancer properties of Antimicrobial peptides -- Using Disease-Associated Enzymes to Activate Antimicrobial Peptide Prodrugs -- Anti-inflammatory Properties of Antimicrobial Peptides and Peptidomimetics: LPS and LTA Neutralization -- Protocols for Screening Antimicrobial Peptides That Influence Virulence Gene Expression in Staphylococcus aureus -- Methods for in vitro Analysis of Antimicrobial Activity and Toxicity of Anti-keratitis Peptides: Bacterial Viability in tears, MTT and TNF-a release assays -- Methods for In Vivo/ex-vivo Analysis of Antimicrobial Peptides in Bacterial Keratitis: siRNA Knockdown, Colony Counts, Myeloperoxidase, Immunostaining, and RT-PCR Assays -- Haemolytic Activity of Antimicrobial Peptides