Title | Skeletal Muscle Regeneration in the Mouse [electronic resource] : Methods and Protocols / edited by Michael Kyba |
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Imprint | New York, NY : Springer New York : Imprint: Springer, 2016 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-3810-0 |
Descript | XII, 346 p. 97 illus., 87 illus. in color. online resource |
Eccentric Contraction-Induced Muscle Injury: Reproducible, Quantitative, Physiological Models to Impair Skeletal Muscle’s Capacity to Generate Force -- Volumetric Muscle Loss -- Freeze Injury of the Tibialis Anterior Muscle -- Synergist Ablation as a Rodent Model to Study Satellite Cell Dynamics in Adult Skeletal Muscle -- Inducing and Evaluating Skeletal Muscle Injury by Notexin and Barium Chloride -- Cardiotoxin Induced Injury and Skeletal Muscle Regeneration -- Fibrosis-Inducing Strategies in Regenerating Dystrophic and Normal Skeletal Muscle -- Isolation, Cryosection, and Immunostaining of Skeletal Muscle -- Isolation of Mouse Periocular Tissue for Histological and Immunostaining Analyses of the Extraocular Muscles and their Satellite Cells -- Skeletal Muscle Tissue Clearing for LacZ and Fluorescent Reporters, and Immunofluorescence Staining -- Isolation, Culture, Functional Assays and Immunofluorescence of Myofiber-Associated Satellite Cells -- Transplantation-Based Quantitative Assays for Satellite Cell Self-Renewal and Differentiation -- Non-Invasive Tracking of Quiescent and Activated MuSC Engraftment Dynamics In Vivo -- Myogenic Progenitors from Mouse Pluripotent Stem Cells for Muscle Regeneration -- Assaying Human Myogenic Progenitor Cell Activity by Reconstitution of Muscle Fibers and Satellite Cells in Immunodeficient Mice -- Methods for Mitochondria and Mitophagy Flux Analyses in Stem Cells of Resting and Regenerating Skeletal Muscle -- Identification, Isolation, and Characterization of Mesenchymal Progenitors in Mouse and Human Skeletal Muscle -- FACS Fractionation and Differentiation of Skeletal-Muscle Resident Multipotent Tie2+ Progenitors -- In Vitro Assays to Determine Skeletal Muscle Physiologic Function -- In Vivo Assessment of Muscle Contractility in Animal Studies -- Functional Measurement of Respiratory Muscle Motor Behaviors Using Transdiaphragmatic Pressure -- Assessment of the Contractile Properties of Permeabilized Skeletal Muscle Fibers -- Analysis of Aerobic Respiration in Intact Skeletal Muscle Tissue by Microplate-Based Respirometry