Title | Rapid Cycle Real-Time PCR โ Methods and Applications [electronic resource] : Genetics and Oncology / edited by Wolfgang Dietmaier, Carl Wittwer, Natarajan Sivasubramanian |
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Imprint | Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 2002 |
Connect to | http://dx.doi.org/10.1007/978-3-642-59397-0 |
Descript | VIII, 205 p. 539 illus., 528 illus. in color. online resource |
for Genetics and Oncology Volume Rapid Cycle Real-Time PCR Methods and Applications -- I Methods Useful in Genetics and Oncology -- Quantification of Cytokine mRNAs in Human Myocardial Biopsy Samples by Real-Time Quantitative PCR Technology Using the LightCycler Instrument -- Quantitative Two-Step RT-PCR for the Detection of Human ABCA1 Transporter on LightCycler Using Hybridization Probes and External Standards -- Quantification of Human Genomic DNA Using Retinoic X Receptor B Gene -- Genotyping by Guanosine-Dependent Quenching of Single-Labeled Fluorescein Probes -- Limitations of Melting Curve Analysis Using SYBR Green I โ Fragment Differentiation and Mutation Detection in the CFTR-Gene -- SYBR Green I Analysis of the Trinucleotide Repeat Responsible for Huntingtonโs Disease -- II Applications in Genetics -- Parallel Genotyping of Different Genes: A Rapid Real-Time PCR Approach -- Detection of a Single Base Substitution in Single Cells by Melting Peak Analysis Using Dual-Color Hybridization Probes -- Rapid Screening for Five Major Cystic Fibrosis Mutations by Melting Peak Analysis Using Fluorogenic Hybridization Probes -- LightCycler PCR for the Polymorphisms โ308 and โ238 in the TNF AIpha Gene and for the TNFB1/B2 Polymorphism in the LT Alpha Gene -- Rapid Genotyping of 2-bp and 9-bp Deletion Mutations Using the LightCycler -- Genotyping of the Methionine-Valine Polymorphism at Codon 129 of the Human Prion Protein by Melting Point Analysis of Fluorescently Labeled Hybridization Probes -- Rapid Detection of Missense Mutations in the Prostatic Steroid 5?-Reductase Gene Using Real-Time Fluorescence PCR and Melting Curve Analysis -- III Applications in Oncolon -- Analysis of Microsatellite Instability by Melting Peak Analysis with BAT26 and BAT25 Specific Fluorescence Hybridization Probes -- Two Color Multiplexing and Typing of Human Papillomavirus Types 16,18 and 45 on LightCycler -- Quantitative Analysis of AML1-ETO Fusion Transcripts in t(8;21) Positive AML Using Real-Time RT-PCR -- Rapid Quantitative Detection of Free Cancer Cells in the Peritoneal Cavity of Gastric Cancer Patients with Real-Time CEA RT-PCR Using Hybridization Probes -- Quantitative Measurement of the mRNA Expression of the Tumor-Associated Antigen PRAME by Real-Time RT-PCR Using LightCycler and SYBR Green I Technology -- Expression Analysis of Telomerase-Genes hTERT and hTR by Quantitative PCR on LightCycler -- Measurement of MDR1 Gene Expression by Real-Time Quantitative RT-PCR Using the LightCycler Instrument