Metallo-β-lactamase (MBL) is the major reason for carbapenems resistance in Pseudomonas aeruginosa and the gene encoding of MBL can be transferred to other bacteria via mobile elements; integron. We aimed to investigate MBL- producing P. aeruginosa strains association with class 1 integron observed in 150 isolates from a 1,200-bed hospital of Thailand. First, we investigated their susceptibility profile by the disc diffusion method and also determined the MIC of imipenem using the E-test. They showed a high rate of resistance to most antibiotics especially quinolones, third-generation cephalosporins, β-lactam/inhibitor and carbapenems. The production of MBL was detected in phenotypic and genotypic by the Modified Hodge Test and PCR. Moreover, class 1-integron genes were detected using multiplex PCR. They showed that 28 isolates were phenotypic MBL positive and presented high-level resistant to imipenem (MIC ≥ 32 μg/ml). In genotypic testing, 33.3% appeared to carry class 1-integron genes. 28 isolates harboured the blaIMP gene and 1 isolate harboured the blaVIM gene and revealed IMP-14 and VIM-2 after nucleotide sequencing. Moreover, IMP-14 producing P. aeruginosa isolates were identical and closely related in PFGE pattern, suggesting that the dissemination of these MBL genes could be due to the clonal dissemination.