Melissococcus plutonius is a widespread bacterial pathogen causing European foulbrood (EFBEFB) disease of honeybee larvae larvae. Recently, MM. plutonius isolates were separated into two types, typical and atypical MM. plutonius , which have different phenotypic and genetic characterization characterization. These could be implied that typical and atypical M. plutonius could have differences in pathogenic mechanisms and various effects on apiculture. In Thailand, EFB can cause extensive losses in both amateur and commercial apiaries. In this study, the native Asian honeybees (Apis cerana, A. florea and A. mellifera) were randomly collected in Thailand. With rapid and feasible technique, we were identified M. plutonius from adult bees without clinical symptoms by PCR assays and determined sequence types (STs) of each isolated M. plutonius using the multilocus sequence typing (MLST).