การศึกษาถึงความแม่นยำของชุดทดสอบบีดีแมกซ์ซีอาร์อีในการทดสอบเชื้อกลุ่มเคล็บเซียลลานิวโมเนียที่มีการสร้างเอนไซม์คาร์บาพีนาเมสจากเสมหะของผู้ป่วยในโรงพยาบาลจุฬาลงกรณ์ / พฤฒิพงศ์ หนูเพชร = Evaluation of the BD MAX tm CRE assay for rapid detection of carbapenemase genes from respirtory secretions of patients infected with Klebsiella pneumoniae in King Chulalongkorn Memorial Hospital (KCMH) / Preudtipong Noopetch
Background: There is a need for rapid detection of carbapenemaseproducing Klebsiella pneumoniae (CPK) for infection control and patient management. The BD MAXTM CRE test is the fully automated platform for detection of resistance genes within 2 h. It has not been validated for respiratory secretion.Objective: To evaluate the performance of the BD MaxTM CRE assay for detection of carbapenemases genes directly from respiratory secretion.Materials and Methods: 169 respiratory secretions, which Klebsiella pneumoniae were isolated from aerobic cultures, were obtained. The BD MAXTM CRE tests were performed to detect carbapenemase genes in respiratory specimens. Laboratory-developed polymerase chain reaction (LDT-PCR) for detection of carbapenemases genes from isolated colony was used as a reference method.Results: When compared with culture followed by LDT-PCR, The BD MAXTM CRE test had 91.12% accuracy, 66.7% sensitivity, and 94.0% specificity.Conclusion: The automated PCR test has an acceptable accuracy with fair sensitivity for the detection of carbapenemase genes from respiratory secretions of patients infected with Klebsiella pneymoniae
