การสกัดและประยุกต์ใช้สารโปรติโอไกลแคนจากกระดูกอ่อนของปลาเพื่อเร่งการหายของบาดแผลไหม้ / ปัญญา วีรวัฒกพงศ์ = Extraction and Application of Proteoglycan from Fish Cartilage for the Treatment of Burn Wounds / Panya Weerawatkapong
Fish cartilage in head part contains epidermal growth factor-like proteoglycan which can stimulate the growth of skin. the objectives of this study were to fabricate silk fibroin (SF) - gelatin (G) scaffold, with and without fish cartilage extracted proteoglycan (E), and to investigate physical, chemical and biological properties of the scaffolds. The composite scaffolds with different blend ratios, with and without the proteoglycan, were fabricated using freeze drying technique. All scaffolds were successfully prepared except SF75G25E. Therefore, SF25G75, SF25G75E, SF50G50 and SF50G50E scaffolds were further characterized for physical, chemical and biological properties. In terms of physical properties, these scaffolds showed porous structures with high pore interconnectivity. SF25G75E scaffold had the largest average pore size. Furthermore, SF25G75 scaffolds had significantly higher water swelling ability than the others. Results from Fourier transform infrared spectroscopy assay showed that the fabricated scaffold containing the extracts had amide and sugar functional group. Regarding biological properties, the scaffold with proteoglycan promoted significantly higher fibroblast cell proliferation rate than those without it. However, the in vitro wound healing was not significantly different with respect to wound closure among all groups. Lastly, the proteoglycan increased biodegradration rate of the scaffold. In conclusion, proteoglycan from fish cartilage was successfully fabricated as the scaffold and had the potential to be used for promoting wound healing by increasing fibroblast cell proliferation.
