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TitleCell Engineering and Regeneration [electronic resource] / edited by Jeffrey M. Gimble, Darja Marolt, Richard Oreffo, Heinz Redl, Susanne Wolbank
ImprintCham : Springer International Publishing : Imprint: Springer, 2019
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Descript 20 illus. online resource


This reference work presents the origins of cells for tissue engineering and regeneration, including primary cells, tissue-specific stem cells, pluripotent stem cells and trans-differentiated or reprogrammed cells. There is particular emphasis on current understanding of tissue regeneration based on embryology and evolution studies, including mechanisms of amphibian regeneration. The book covers the use of autologous versus allogeneic cell sources, as well as various procedures used for cell isolation and cell pre-conditioning , such as cell sorting, biochemical and biophysical pre-conditioning, transfection and aggregation. It also presents cell modulation using growth factors, molecular factors, epigenetic approaches, changes in biophysical environment, cellular co-culture and other elements of the cellular microenvironment. The pathways of cell delivery are discussed with respect to specific clinical situations, including delivery of ex vivo manipulated cells via local and systemic routes, as well as activation and migration of endogenous reservoirs of reparative cells. The volume concludes with an in-depth discussion of the tracking of cells in vivo and their various regenerative activities inside the body, including differentiation, new tissue formation and actions on other cells by direct cell-to-cell communication and by secretion of biomolecules


Cells for Cartilage Regeneration -- Primary Cells: Periosteal Cells -- Stem Cells: Bone Marrow Derived - MSC and HSC -- Embryonic Stem Cells -- Induced Pluripotent Stem Cells and Transdifferentiation -- Stem Cells – Aging Aspects -- Biophysical Preconditioning and Modulation - Low Level Light Therapy (LLLT) in Wound Healing" -- Biophysical Preconditioning and Modulation -- miRNA Modulation -- Cell Storage – Activity Control -- Challenges and Status of Adipose Cell Therapies: Translation and Commercialization -- Peripheral Blood Mononuclear Cell Secretome for Tissue Repair -- Systemic Applications -- Cell Culture Conditions - Cultivation of Stem Cells under Dynamic Conditions -- Mesenchymal Stem Cell Secretome for Tissue Repair -- Cell Culture Conditions – Cell Derived Factors -- Cell Differentiation -- Primary Cells - overview of Different Cell Types Contributing to Muscle Repair and Regeneration -- Stem Cells: Umbilical Cord/Wharton’s Jelly Derived -- Use of Xenogeneic Cells -- Material Based Preconditioning and Modulation (Molecules, Patterns and Surface Hardness) -- Cell Aggregates - Sheets -- Epigenetic Modulation Approaches -- Cell Tracking -- Stem Cells: Olfactory Mucosa -- Basics of Self-Regeneration -- Translating from Animal Systems (Models) to Human Cells -- Basics of Amphibian Self-Regeneration -- Biological Augmentation for Tendon Repair – Lessons to be Learned from Development, Disease, and Tendon Stem Cell Research -- Primary Cells: Liver Cells -- Stem Cells: Amniotic Fluid -- Adipose Derived Stem Cells -- Stem Cells: Perivascular Origin -- Cell Sorting -- Immortalized Primary Cells for Test Systems -- Cell Transfection -- Molecular Crowding – (in Cell Culture) -- Primary Cells: Pancreatic Cells -- Primary Cells: Schwann Cells and Neurons -- Human Amniotic Membrane Cells: Basic Characteristics -- Stem Cells: Skin -- Blood Derived Stem Cells -- Use of Autologous or Allogeneic Cells -- Cell Tools for Mechanistic Studies -- Primary Cells: Keratinocytes and Skin Fibroblasts -- Pluripotent Stem Cells from Different Sources -- Muse Cells -- Biochemical Preconditioning and Modulation -- Cell Sheets -- Two Level Approaches -- Cell-Cell Communication by Exosomes and Microvesicles -- Local Applications -- Endogenous Cell Catching -- Cell-Cell Contact -- Activation and Differentiation of Endogenous Stem Cells During a Wound Healing -- Needs for Clinical Application of Stem Cells -- Regulatory Aspects of Cell Therapy.-

Biomedical engineering Biomaterials Biomedical Engineering and Bioengineering. Biomaterials. Regenerative Medicine/Tissue Engineering. Biological and Medical Physics Biophysics.


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