I: Laboratory procedures -- The documentation system -- Culture media techniques -- IVF incubator handling -- Preparation for follicle aspiration and isolation of cumulus-oocyte-complexes (COC) -- Semen collection and sample reception -- Spermiogramm, makler chamber, neubauer chamber, CASA -- Sperm viability testing -- Sperm preparation–swim up, density gradient, migration chamber, zeta method -- Sperm selection using hyaluronan binding -- IVF–insemination -- ICSI -- Oocyte in vitro maturation–isolation of COCs and culture for maturation -- Artificial oocyte activation -- Cryopreservation–sperm TESE and MESA -- Vitrification–oocytes, 2PN, embryos, blastocysts -- Slow freezing: principles, oocytes, 2PN, embryos, blastocysts -- Oocyte selection -- Zygote selection -- Selection and grading of embryos on day 2/3 and blastocysts on day 5/6 -- Time-lapse imaging -- Assisted hatching -- Zona manipulation at various embryo stages: mechanical/acidic medium -- Biopsy: polar body, blastomere, trophectoderm -- Sampling of cells after biopsy for aCGH -- Embryo transfer–standard and ultrasound guided -- Hygenic standards in the IVF laboratory -- Quality control of IVF laboratory supplies–mouse embryo assay (MEA) -- Troubleshooting in the IVF laboratory -- Microscopy -- Fertility preservation -- II: Clinical procedures -- Ultrasound evaluation in treatment management -- Three-dimensional ultrasound and the diagnosis of uterine anomalies -- Infertility ultrasound evaluation: one stop procedure -- Office hysteroscopy -- Hysteroscopic management of uterine septum -- Hysteroscopic lysis of intrauterine adhesions -- Hysteroscopic management of submucosal fibroids -- Saline infusion sonohysterography -- General principles of laparoscopy -- Microlaparoscopy -- Laparoscopic myomectomy and minilaparotomy: an alternative approach for myomectomy -- Laparoscopic excision of a large endometrioma -- Laparoscopic management of subtle fimbrial pathology secondary to endometriosis -- Laparoscopic management of mature cystic teratoma -- Tubal cannulation for proximal tubal disease -- Robotic assisted laparoscopic myomectomy -- Robotic assisted tubal anastomosis after tubal sterilization -- Unexplained infertility -- Male evaluation for infertility -- Hysterosalpingography -- Intrauterine insemination: clinical perspectives -- GnRH agonist to trigger final follicular maturation in IVF -- GnRH agonist protocols -- GnRH agonists before IVF for advanced endometriosis -- Microdose GnRH agonist flare protocols -- Gonadotropin releasing hormone antagonist cycle for in vitro fertilization -- Minimal stimulation for IVF -- Ovarian hyperstimulation syndrome -- The prediction of OHSS -- Tips on technique of embryo transfer -- Difficult embryo transfers -- In vitro maturation of oocytes for fertility treatment and fertility preservation -- Complications and outcomes of IVF -- Obstetric complications after in-vitro fertilization -- Twins pregnancy after ART -- IVF and endometriosis -- Leiomyoma of the uterus -- Office management of polycystic ovarian syndrome -- Adenomyosis: imaging and treatment -- Ectopic pregnancy: evaluation and management

1. Fertilization in Vitro -- methods
2. Embryo Culture Techniques -- methods
3. Pregnancy Complications -- prevention & control