IVF : the first four decades -- Evaluation and preparation of the infertile couple for in vitro fertilization -- Carrier screening -- Testing ovarian reserve -- Basics of ovarian stimulation -- Role of antagonists in human IVF : the tool to a safer and more patient friendly IVF -- Ultrasonography in IVF -- Oocyte retrieval -- In vitro maturation for clinical use -- Sperm preparation for IVF and ICSI -- Assisted fertilization -- Analysis of fertilization -- Human embryo development and assessment of viability -- Embryo culture systems -- Polar body, cleavage stage and trophectoderm biopsy -- Pre-implantation genetic testing -- Oocytes and embryo cryopreservation -- Quality control and quality assurance in the IVF laboratory -- Oocyte donation -- Endometrial receptivity -- Embryo transfer -- Multiple vs singleton births : consequences and prevention -- Monitoring and evaluation of infertility treatments : is there a priority for monitoring of cognitive function in children born from ART and other treatments?