This study aimed to develop the tablet formulation of Serratia sp. W4-01 for lipid degradation in wastewater. Serratia sp. W4-01 could degrade various types of lipids such as soybean oil, palm oil, rice-bran oil, sunflower oil, olive oil and lard at concentration 10 g/l within 3 days and the gene encoding for lipase enzyme (lipA) involved in lipid degradation could be detected in strain W4-01. Therefore, the tablet formulation was developed and the formulation that have the highest lipid degradation efficiency consisted of lyophilized bacteria containing skim milk and monosodium glutamate, ammonium sulfate, potassium dihydrogen phosphate, sucrose, magnesium stearate and maize flour. The tablet could store in an anaerobic condition at 4℃ up to 6 months with 63% of survival rate while still retain its lipid removal ability. The tablet showed lipid removal efficacy of higher or comparable to those of commercial products. The W4-01 tablet was tested for its lipid-contaminated wastewater treatment in a grease trap model carried out continuously. The total volume of model was 4 liters with a hydraulic retention time of 12 hours with W4-01 addition daily. The tablet was able to treat wastewater from coffee shop containing 350 mg/l of lipid with 70% lipid removal within 21 days. In addition, the treated wastewater showed no toxic effect toward the growth of green bean, radish and cucumber seed. This is the pioneer research for the development of tablet formulation with pure culture of Gram-negative bacteria for lipid-contaminated wastewater treatment. The results indicated that the tablet form of W4-01 has potential to use in large scale of lipid-contaminated wastewater treatment.
