Title | Advanced Methods in Structural Biology [electronic resource] / edited by Toshiya Senda, Katsumi Maenaka |
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Imprint | Tokyo : Springer Japan : Imprint: Springer, 2016 |
Connect to | http://dx.doi.org/10.1007/978-4-431-56030-2 |
Descript | X, 340 p. 96 illus., 65 illus. in color. online resource |
Part 1. Protein expression -- 1 Expression in Bacteria and Refolding -- 2 Expression of proteins in insect and mammalian cells -- 3 Application of MultiBac system to large complexes -- 4 Purification using affinity-tag technology -- 5 Cell-Free Protein Production for Structural Biology -- Part 2. Purification and crystallization of membrane proteins -- 6 Overview of membrane protein purification and crystallization -- 7 Cell-free synthesis of membrane proteins -- Part 3. Crystallization and Crystal Engineering -- 8 Screening of cryoprotectants and the multistep soaking method -- 9 Protein modification for crystallization -- Part 4. Interaction analysis -- 10 Analytical ultracentrifugation -- 11 Mass spectrometry -- 12 Frontal gel filtration -- 13 Surface Plasmon Resonance -- Part 5. Advanced methods for structural analyses -- 14 Structural biology with micro-focus beam-lines -- 15 Structural biology and electron microscopy -- 16 Structure determination software for macromolecular X-ray crystallography -- 17 NMR Structural Biology using Paramagnetic Lanthanide Probe