Title | Skeletal Development and Repair [electronic resource] : Methods and Protocols / edited by Matthew J. Hilton |
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Imprint | Totowa, NJ : Humana Press : Imprint: Humana Press, 2014 |
Connect to | http://dx.doi.org/10.1007/978-1-62703-989-5 |
Descript | X, 328 p. 78 illus., 40 illus. in color. online resource |
Overview of Skeletal Development -- Overview of Skeletal Repair (Fracture Healing and Its Assessment) -- Generation of Closed Transverse Fractures in Small Animals -- The Murine Femoral Bone Graft Model and a Semi-Automated Histomorphometric Analysis Tool -- Surgical Induction of Posttraumatic Osteoarthirits -- A Mouse Model of Flexor Tendon Repair -- Cartilage Explant Cultures -- Renal Capsule Transplantations to Assay Skeletal Angiogenesis -- Whole Mount Skeletal Staining -- Tips and Techniques for Processing and Sectioning Undecalcified Murine Bone Specimens -- Preparation of Thin Sections from Non-fixed and Undecalcified Hard Tissues Using Kawamoto’s Film Method (2012) -- Demineralized Murine Skeletal Histology -- Beta-Galactosidase Staining in the Skeleton -- Whole-Mount In Situ Hybridization on Murine Skeletogenic Tissues -- Non-Radioactive In Situ Hybridization on Skeletal Tissue Sections -- Radioactive In Situ Hybridization to Detect Gene Expression in Skeletal Tissue Sections -- Proliferation Assays (BrdU and EdU) on Skeletal Tissue Sections -- TUNEL Assay on Skeletal Tissue Sections to Detect Apoptosis -- Analysis of Chondrogenesis Using Micromass Cultures of Limb Mesenchyme -- Isolation and Culture of Murine Primary Chondrocytes -- Bone Marrow Stromal Cell Assays – In Vitro and In Vivo -- Isolation and Culture of Neonatal Mouse Calvarial Osteoblasts -- RANKL-Based Osteoclastogenic Assays from Murine Bone Marrow Cells -- Hematopoietic Stem Cell Cultures and Assays