Title | Environmental Microbiology [electronic resource] : Methods and Protocols / edited by Ian T. Paulsen, Andrew J. Holmes |
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Imprint | Totowa, NJ : Humana Press : Imprint: Humana Press, 2014 |
Edition | 2nd ed. 2014 |
Connect to | http://dx.doi.org/10.1007/978-1-62703-712-9 |
Descript | X, 242 p. 35 illus., 19 illus. in color. online resource |
Methods for Isolation and Cultivation of Filamentous Fungi -- Rapid Extraction of PCR-Competent DNA from Recalcitrant Environmental Samples -- Quantitative PCR for Detection of mRNA and gDNA in Environmental Isolates -- Analysis of Community Dynamics in Environmental Samples Using Denaturing Gradient Gel Electrophoresis -- Terminal Restriction Fragment Length Polymorphism (T-RFLP) Profiling of Bacterial 16S rRNA Genes -- Profiling the Diversity of Microbial Communities with Single-Strand Conformation Polymorphism (SSCP) -- Human Fecal Source Identification with Real-Time Quantitative PCR -- Next Generation Barcode Tagged Sequencing for Monitoring Microbial Community Dynamics -- Analysis of Methanotroph Community Structure Using a pmoA-Based Microarray -- Biolog Phenotype MicroArrays for Phenotypic Characterization of Microbial Cells -- Visualization of Metabolic Properties of Bacterial Cells Using Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS) -- Single-Cell Raman Sorting -- Bacterial Whole Cell Biosensors for the Detection of Contaminants in Water and Soils -- Stable Isotope Probing to Study Functional Components of Complex Microbial Ecosystems -- Metagenomics Using Next-Generation Sequencing -- Targeted Genomics of Flow Cytometrically Sorted Cultured and Uncultured Microbial Groups -- Quantitative Microbial Metatranscriptomics -- Quantitative Metaproteomics: Functional Insights into Microbial Communities