Title | PIWI-Interacting RNAs [electronic resource] : Methods and Protocols / edited by Mikiko C. Siomi |
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Imprint | Totowa, NJ : Humana Press : Imprint: Humana Press, 2014 |
Connect to | http://dx.doi.org/10.1007/978-1-62703-694-8 |
Descript | XI, 249 p. 48 illus., 23 illus. in color. online resource |
Chromatin Immunoprecipitation Assay of Piwi in Drosophila -- Drosophila Germline Stem Cell for in vitro Analyses of PIWI-Mediated RNAi -- RNAi and Overexpression of Genes in Ovarian Somatic Cells -- Making piRNAs in vitro -- A Framework for piRNA Cluster Manipulation -- Mass Spectrometric Analysis of 3’-End Methylation of piRNAs -- HITS-CLIP (CLIP-Seq) for Mouse Piwi Proteins -- Analysis of DNA Methylation in Mouse Testes -- Analysis of Small RNA-Guided Endonuclease Activity in Endogenous Piwi Protein Complexes from Mouse Testes -- Small RNA Library Construction from Minute Biological Samples -- Analysis of sDMA Modifications of PIWI Proteins -- Analyses of piRNA-Mediated Transcriptional Transposon Silencing in Drosophila: Nuclear Run-On Assay on Ovaries -- Combined RNA/DNA Fluorescence in situ Hybridization on Whole-Mount Drosophila Ovaries -- Fast and Accurate Method to Purify Small Non-Coding RNAs from Drosophila Ovaries -- Isolation of Zebrafish Gonads for RNA Isolation -- Small RNA Library Construction for High-Throughput Sequencing -- Analysis of Piwi-Loaded Small RNAs in Tetrahymena -- Effective Gene Knockdown in the Drosophila Germline by Artificial miRNA-Mimicking siRNAs -- Isolation and Bioinformatics Analyses of Small RNAs Interacting with Germ-Cell Specific Argonaute in Rice