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TitleImmunoproteomics [electronic resource] : Methods and Protocols
Author edited by Kelly M. Fulton, Susan M. Twine
ImprintTotowa, NJ : Humana Press : Imprint: Humana Press, 2013
Connect tohttp://dx.doi.org/10.1007/978-1-62703-589-7
Descript XI, 370 p. 73 illus., 33 illus. in color. online resource

SUMMARY

Providing current diverse approaches and techniques used to study the immunoproteome, Immunoproteomics: Methods and Protocols collects chapters from key researchers that deliver information to be used in diagnostics, disease progression, and vaccine correlates of protection analysis, to name but a few. This detailed volume includes techniques used for the study of the antibody targets of bacterial pathogens, viruses, and cancer, mass spectrometry-based approaches to characterize T-cell epitopes, chapters on detection and relative quantification of cytokines in serum, as well as in silico prediction of epitopes using sequence-based or modeling approaches.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and thorough, Immunoproteomics: Methods and Protocols aids researchers in transferring these techniques to their own laboratories in addition to providing a reference to guide researchers toward appropriate techniques


CONTENT

Introduction to the Immune System -- Immunoproteomics: Current Technology and Applications -- Antigen Identification Using SEREX -- Antigen Discovery Using Whole-Genome Phage Display Libraries -- Methods and Applications of Serological Proteome Analysis -- Pre-Absorbed Immunoproteomics: A Novel Method for the Detection of Bacterial Surface Proteins -- Identification of the Antigen Content of Electroimmunoprecipitates -- An Immunoproteomics Approach to Screen the Antigenicity of the Influenza Virus -- In Vivo Microbial Antigen Discovery (InMAD) to Identify Diagnostic Proteins and Polysaccharides that Are Circulating During Microbial Infections -- Chemo-Enzymatic Production of O-Glycopeptides for the Detection of Serum Glycopeptide Antibodies -- Enrichment and Characterization of Glycopeptide Epitopes from Complex Mixtures -- Whole-Cell MALDI-TOF Mass Spectrometry: A Tool for Immune Cell Analysis and Characterization -- Cell-Based Arrays for the Identification of Interacting Polypeptide Domains or Epitopes -- Identification and Quantitation of MHC Class II-Bound Peptides from Mouse Spleen Dendritic Cells by Immunoprecipitation and Mass Spectrometry Analysis -- Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification -- Profiling of Cytokine and Chemokine Responses Using Multiplex Bead Array Technology -- Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis -- Identification of Protein Biomarkers in Human Serum Using iTRAQ and Shotgun Mass Spectrometry -- Genome-Based Bioinformatic Prediction of Major Histocompatibility Complex (MHC) Epitopes -- Structure-Based Prediction of Major Histocompatibility Complex (MHC) Epitopes -- Prioritization of Therapeutic Targets of Inflammation Using Proteomics, Bioinformatics, and In Silico Cell-Cell Interactomics -- Commercial Considerations for Immunoproteomics


Medicine Immunology Proteomics Biomedicine Immunology Proteomics



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