Title | Adhesion Protein Protocols [electronic resource] / edited by Amanda S. Coutts |
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Imprint | Totowa, NJ : Humana Press : Imprint: Humana Press, 2013 |
Edition | 3rd ed. 2013 |
Connect to | http://dx.doi.org/10.1007/978-1-62703-538-5 |
Descript | XII, 407 p. 87 illus., 57 illus. in color. online resource |
Reconstructing Integrin Activation in vitro -- Quantifying Cellular Adhesion to Covalently Immobilized Extracellular Matrix Proteins by Single-Force Spectroscopy -- A Multimode-TIRFM & Microfluidic Technique to Examine Platelet Adhesion Dynamics -- Localization-Based Super-Resolution Imaging of Cellular Structures -- Use of Microarray Analysis to Investigate EMT Gene Signatures.- Podosome Reformation in Macrophages: Assays and Analysis -- Mobile and Three-Dimensional Presentation of Adhesion Proteins Within Microwells -- Basement Membrane Invasion Assays: Native Basement Membrane and Chemoinvasion Assay -- Cytoplasmic Actin: Purification and Single Molecule Assembly Assays -- Synthetic and Tissue-Derived Models for Studying Rigidity Effects on Invadopodia Activity -- Determining Rho GTPase Activity by an Affinity-Precipitation Assay -- Proteomic and Biochemical Methods to Study the Cytoskeletome. Fabricating Surfaces with Distinct Geometries and Different Combinations of Cell Adhesion Proteins -- Purification of Native Arp2/3 Complex from Bovine Thymus -- Purification of Arp2/3 Complex from Saccharomyces Cerevisiae -- Measurement and Analysis of in vitro Actin Polymerization -- Use of the xCELLigence System for Real Time Analysis of changes in Cellular Motility and Adhesion in Physiological Conditions -- Measuring Chemotaxis Using Direct Visualisation Microscope Chambers -- In Vitro Microtubule Severing Assays -- VE-Cadherin Status as an Indicator of Microvascular Permeability -- High-Resolution Live-Cell Imaging and Time-Lapse Microscopy of Invadopodium Dynamics and Tracking Analysis -- Live Cell Imaging of RhoGTPase Biosensors in Tumor Cells -- Electrospun Nanofiber Scaffolds for Investigating Cell-matrix Adhesion -- Method for Measuring Single-Molecule Adhesion Forces and Attachment Lifetimes of Protein-Membrane Interactions -- VE-Cadherin Status as an Indicator of Microvascular Permeability -- High-Resolution Live-Cell Imaging and Time-Lapse Microscopy of Invadopodium Dynamics and Tracking Analysis. Live Cell Imaging of RhoGTPase Biosensors in Tumor Cells -- Electrospun Nanofiber Scaffolds for Investigating Cell-matrix Adhesion -- Method for Measuring Single-Molecule Adhesion Forces and Attachment Lifetimes of Protein-Membrane Interactions