Title | Transplantation Immunology [electronic resource] : Methods and Protocols / edited by Andrea A. Zachary, Mary S. Leffell |
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Imprint | Totowa, NJ : Humana Press : Imprint: Humana Press, 2013 |
Edition | 2nd ed. 2013 |
Connect to | http://dx.doi.org/10.1007/978-1-62703-493-7 |
Descript | XIII, 411 p. 49 illus., 29 illus. in color. online resource |
Alloreactivity -- Antibodies in Transplantation: The Effects of HLA and Non-HLA Antibody Binding and Mechanisms of Injury -- Cell Mediated Rejection -- Transplantation Tolerance -- Composite Tissue Transplantation -- Transplantation of the Sensitized Patient: Histocompatibility Testing -- Thoracic Organ Transplantation: Laboratory Methods -- HLA Typing by Sequence Specific Primers -- Human Leukocyte Antigen (HLA) Typing By DNA Sequencing -- Next-Generation HLA Sequencing using the 454 GS FLX System -- Chimerism Testing by Quantitative PCR Using Indel Markers -- Gene-specific PCR Typing of Killer Cell Immunoglobulin-like Receptors (KIR) -- Complement-dependent Cytotoxicity Crossmatch -- Lymphocyte Crossmatching by Flow Cytometry -- Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA Specific Antibodies -- C1q Assay for the Detection of Complement Fixing Antibody to HLA Antigens -- Tetramer Staining for the Detection of HLA-specific B cells -- A Flow Cytometric Crossmatch Test Using Endothelial Precursor Cells Isolated from Peripheral Blood -- Detection of Antibodies to Self-antigens (K-alpha 1 Tubulin, Collagen I, II, IV and V Myosin and Vimentin) by Enzyme-Linked Immunosorbent Assay (ELISA) -- Cylex ImmuKnow Cell Function Assay -- Detection of Intracellular Cytokines -- Tolerogenic Dendritic Cells and Induction of T Suppresson Cells in Transplant Recipients -- Discovery and Customized Validation of Antibody Targets by Protein Arrays and Indirect ELISA -- RNA Purification and Expression Analysis using Microarrays and RNA Deep Sequencing