Title | Nanomaterial Interfaces in Biology [electronic resource] : Methods and Protocols / edited by Paolo Bergese, Kimberly Hamad-Schifferli |
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Imprint | Totowa, NJ : Humana Press : Imprint: Humana Press, 2013 |
Connect to | http://dx.doi.org/10.1007/978-1-62703-462-3 |
Descript | XI, 293 p. 73 illus., 53 illus. in color. online resource |
Preparation of 2 nm Gold Nanoparticles for In vitro and In vivo Applications -- DNA Conjugation to Nanoparticles -- Conjugation of Nanoparticles to Proteins -- Water-Solubilization and Functionalization of Semiconductor Quantum Dots -- Synthesizing and Modifying Peptides for Chemoselective Ligation and Assembly into Quantum Dot-Peptide Bioconjugates -- Reliable Methods for Silica Coating of Au Nanoparticles -- Surface Modifications by Polymers for Biomolecule Conjugation -- Functionalization Protocols of Silicon Micro-Nano-Mechanical Biosensors -- Stability and Aggregation Assays of Nanoparticles in Biological Media -- Electrochemical Measurements of DNA Melting on Surfaces -- Formation and Characterization of Nanoparticle-Protein Corona -- Electrophoretic Implementation of the Solution-Depletion Method for Measuring Protein Absorption, Adsorption Kinetics, and Adsorption Competition Among Multiple Proteins in Solution -- Hyperspectral Microscopy for Characterization of Gold Nanoparticles in Biological Media and Cells for Toxicity Assessment -- Immunochemistry, Electron Tomography and Energy Dispersive X-Ray Spectroscopy (EDXS) on Cryosections of Human Cancer Cells Doped with Stimuli Responsive Polymeric Nanogels Loaded with Iron Oxide Nanoparticles -- Zwitterion Siloxane to Passivate Silica Against Non-Specific Protein Adsorption -- Preparation and Characterization of DNA Block Copolymer Assemblies Loaded with Nanoparticles -- Polyaspartic Acid Coated Iron Oxide Nanoprobes for PET/MRI Imaging -- Ligand Synthesis and Passivation for Silver and Large Gold Nanoparticles for Single-Particle-Based Sensing and Spectroscopy -- Non-Covalent Intracellular Drug Delivery of Hydrophobic Drugs on Au NPs -- Modification of Carbon Nanotubes for Gene Delivery Vectors -- Lipid-Based Nanoparticles as Non-Viral Gene Delivery Vectors -- Stabilizing Gold Nanoparticle Bioconjugates in Physiological Conditions by PEGylation