Title | Neural Development [electronic resource] : Methods and Protocols / edited by Renping Zhou, Lin Mei |
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Imprint | Totowa, NJ : Humana Press : Imprint: Humana Press, 2013 |
Connect to | http://dx.doi.org/10.1007/978-1-62703-444-9 |
Descript | XIII, 347 p. 59 illus., 48 illus. in color. online resource |
Isolation and Maintenance of Cortical Neural Progenitor Cells In Vitro -- Neural Induction with a Dopaminergic Phenotype from Human Pluripotent Stem Cells Through a Feeder-Free Floating Aggregation Culture -- Nucleoside Analog Labeling of Neural Stem Cells and Their Progeny -- Culture of Dissociated Hippocampal Neurons -- Culturing Mouse Cerebellar Granule Neurons -- Preparation of Primary Cultured Dopaminergic Neurons from Mouse Brain -- Culturing Astoryctes from Postnatal Rats -- Culturing Oligodendrocyte Lineage Cells from Neonatal Rats -- Isolation and Culture of Schwann Cells -- DNA Transfection: Calcium Phosphate Method -- The Gene-Gun Approach for Transfection and Labeling of Cells in Brain Slices -- Lentiviral Vector Production, Titration, and Transduction of Primary Neurons -- In-Ovo Electroporation in Embryonic Chick Spinal Cords -- Gene Transfer in Developing Chick Embryos: In Ovo Electroporation -- In Utero Electroporation in Mice -- General Introduction to In Situ Hybridization Protocol Using Non-Radioactively Labeled Probes to Detect mRNAs on Tissue Sections -- Immunofluorescence Staining with Frozen Mouse or Chick Embryonic Tissue Sections -- β-Galactosidase Staining of LacZ Fusion Proteins in Whole Tissue Preparations -- Chromatin Immunoprecipitation Assay of Brain Tissues Using Percoll Gradient-Purified Nuclei -- In Vivo Dual Luciferase Reporter Assay with Chick Neural Tube In Ovo Electroporation System -- Growth Cone Collapse Assay -- The Stripe Assay: Studying Growth Preference and Axon Guidance on Binary Choice Substrates In Vitro -- Microcontact Printing of Substrate-Bound Protein Patterns for Cell- and Tissue Culture -- Semi-Automated Analysis of Dendrite Morphology in Cell Culture -- Monitoring Synaptic Plasticity by Imaging AMPA Receptor Content and Dynamics on Dendritic Spines -- Morphological Analysis of Neuromuscular Junctions by Immunofluorescent Staining of Whole-Mount Mouse Diaphragms- Routine Histology Techniques for the Developing and Adult Central Nervous System -- Cryosectioning -- The Golgi-Cox Method -- Neuroanatomical Tract-Tracing Using Biotinylated Dextran Amine -- Retrograde Tracing Technique for Neonatal Animals