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TitleArray Comparative Genomic Hybridization [electronic resource] : Protocols and Applications
Author edited by Diponkar Banerjee, Sohrab P. Shah
ImprintTotowa, NJ : Humana Press : Imprint: Humana Press, 2013
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Descript XII, 382 p. 33 illus., 16 illus. in color. online resource


The earliest descriptions of human chromosomes initiated the genomics revolution that is now upon us. Array Comparative Genomic Hybridization: Protocols and Applications explores the scope of what is now possible as far as documenting abnormalities associated with several human cancers. While the technology for interrogating the human genome continues to evolve, refinement of comparative genomic hybridization (CGH) using array CGH and related technologies have provided enormous insight into human cancers at an affordable scale. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Array Comparative Genomic Hybridization: Protocols and Applications provides researchers with well-honed methodologies to learn these techniques for their own use in research or clinical diagnostic laboratories


Array Comparative Genomic Hybridization: An Overview of Protocols, Applications and Technology Trends -- Ultra Dense Array CGH and Discovery of Micro-Copy Number Alterations and Gene Fusions in the Cancer Genome -- Epigenomics: Sequencing the Methylome -- Application of Array Comparative Genomic Hybridization in Chronic Myeloid Leukemia -- The Use of Cytogenetic Microarrays in Myelodysplastic Syndrome Characterization -- CGH Protocols - Chronic Lymphocytic Leukemia -- Analysis of Acquire Genomic Copy Number Aberrations and Regions of Loss of Heterozygosity in Acute Myelogenous Leukemia Fenomes using Affymetrix SNP 6.0 Arrays and Supporting Software Tools -- Clinical Applications of BAC Array-CGH to the Study of Diffuse Large B-Cell Lymphomas -- Genomic Profiling of Mantle Cell Lymphoma -- Copy Number Analysis in EBV-Positive Nodal Peripheral T-Cell Lymphoma, Unspecified -- Mycosis Fungoides and Sézary Syndrome -- Array CGH Reveals Clonal Evolution of Adult T Cell Leukemia/Lymphoma -- Array-CGH Analysis of Cutaneous Anaplastic Large Cell Lymphoma -- Genome Wide DNA-Profiling of HIV-Related B-Cell Lymphomas -- Array Comparative Genomic Hybridization in Osteosarcoma -- Comparative Genomic Hybridization of Wilms' Tumor -- Array-CGH in Childhood MDS -- Non-Familial Breast Cancer Subtypes -- Applications of Array-CGH for Lung Cancer -- Array CGH in Brain Tumours -- Finding Common Regions of Alteration in Copy Number Data -- Distinguishing Somatic and Germline Copy Number Events in Cancer Patient DNA Hybridized to Whole Genome SNP Genotyping Arrays

Medicine Human genetics Laboratory medicine Biomedicine Human Genetics Laboratory Medicine


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