Title | p53 Protocols [electronic resource] / edited by Sumitra Deb, Swati Palit Deb |
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Imprint | Totowa, NJ : Humana Press : Imprint: Humana Press, 2013 |
Edition | 2nd ed. 2013 |
Connect to | http://dx.doi.org/10.1007/978-1-62703-236-0 |
Descript | X, 238 p. 51 illus., 33 illus. in color. online resource |
Detecting and Quantifying p53 Isoforms at mRNA Level in Cell Lines and Tissues -- Detecting p53 Isoforms at Protein Level -- Autophagy, Senescence and Apoptosis -- p53 and Cell Cycle Effects after DNA Damage -- p53 Ubiquitination and Proteasomal Degradation.-Identification of p53 in Mitochondria.-Identification of Novel Mutant p53 Interacting Proteins by Proteomic Analysis.-Identification of Small Molecules Affecting p53-MDM2/MDMX Interaction by Fluorescence Polarization -- Determine the Effect of p53 on Chemosensitivity.-Measurement of Chemosensitivity and Growth Rate in p53 Expressing Cells -- Mutant p53 in Cell Adhesion and Motility -- Use of the DNA Fiber Spreading Technique to Detect the Effects of Mutant p53 on DNA Replication -- Generation of p53-deficient Induced Pluripotent Stem Cells (iPS) from Mouse Embryo Fibroblasts -- p53 Actions on MicroRNA Expression and Maturation Pathway -- Isolation and Characterization of Murine Multipotent Lung Stem Cells -- Generation of p53 Knock-down Cell Lines.-ChIP for Identification of p53 Responsive DNA Promoters -- ChIP-on-chip to Identify Mutant p53 Targets -- ChIP Sequencing to Identify p53 Targets -- The p53-Mdm2 Loop: A Critical Juncture of Stress Response