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TitleMolecular Dermatology [electronic resource] : Methods and Protocols
Author edited by Cristina Has, Cassian Sitaru
ImprintTotowa, NJ : Humana Press : Imprint: Humana Press, 2013
Connect tohttp://dx.doi.org/10.1007/978-1-62703-227-8
Descript XII, 460 p. 84 illus., 59 illus. in color. online resource

SUMMARY

The sustained skin research efforts over the past decades has led to the accumulation of a significant collection of information on skin structure and physiology as well as on the pathogenesis of cutaneous diseases.  In Molecular Dermatology: Methods and Protocols, leading experts in the field provide a collection of state-of-the-art reliable protocols covering a wide spectrum of techniques and experimental models, specific molecular assays and disease models, as well as overviews of diagnostic and research areas relevant to molecular dermatology.  As a volume in the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Comprehensive and authoritative, Molecular Dermatology: Methods and Protocols emphasizes the vital importance of skin research and collects the methodologies necessary to aid scientists in moving forward in this valuable field


CONTENT

Molecular Dermatology Comes of Age -- Molecular Diagnosis of Autoimmune Blistering Diseases -- Molecular Diagnosis of Genodermatoses -- Three-Dimensional Visualization of the Molecular Architecture of Cell-Cell Junctions In Situ by Cryo-Electron Tomography of Vitreous Sections -- 3D Visualization of Epidermal Langerhans Cells -- In Vivo Imaging of Lymph Node Lymphangiogenesis by Immuno-Positron Emission Tomography -- Optical Imaging of HPV Infection in a Murine Model -- Laser Scanning Microscopy Approach for Semiquantitation of In Vitro Dermal Particle Penetration -- Analysis of Cutaneous Somatic Mosaicism -- Global Proteome Analyses of SILAC Labeled Skin Cells -- MicroRNA Profiling During Human Keratinocyte Differentiation Using a Quantitative Real Time PCR Method -- Cell Death in the Skin: How to Study Its Quality and Quantity? -- In Vitro Pathogenicity Assay for Anti-Desmoglein Autoantibodies in Pemphigus -- Induction of Granulocyte-Dependent Dermal-Epidermal Separation by Autoantibodies Ex Vivo -- Analysis of Collective Invasion of Carcinoma Cells in a 3D Organotypic Model -- Isolation of Melanoma Tumor Initiating Cells from Surgical Tissues -- Detection, Enumeration, and Characterization of Immune Cells Infiltrating Melanoma Tumors -- Analysis of Cell Movement Between Skin and Other Anatomical Sites In Vivo Using Photoconvertible Fluorescent Protein “Kaede”-Transgenic Mice -- Reconstitution of Skin Fibrosis Development Using a Tissue Engineering Approach -- Bioengineered Skin Humanized Model of Psoriasis -- Induction of Contact Hypersensitivity in the Mouse Model -- Generation of Functional Multipotent Keratinocytes from Mouse Induced Pluripotent Stem Cells -- RNAi-Mediated Gene Function Analysis in Skin -- Mouse Models of Autoimmune Blistering Diseases Induced by the Passive Transfer of Antibodies -- Induction of Experimental Epidermolysis Bullosa Acquisita by Immunization with Murine Collagen VII -- Animal Model for Cutaneous Leishmaniasis -- Model for Generation of Large Numbers of Primary, Inflammatory Skin-Derived Neutrophils and Macrophages -- Zebrafish as a Model System to Study Heritable Skin Diseases -- Cell- and Protein-Based Therapy Approaches for Epidermolysis Bullosa -- RNA Trans-Splicing for Genodermatoses


Medicine Molecular biology Endocrinology Biomedicine Molecular Medicine Endocrinology



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