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TitleMolecular Dermatology [electronic resource] : Methods and Protocols
Author edited by Cristina Has, Cassian Sitaru
ImprintTotowa, NJ : Humana Press : Imprint: Humana Press, 2013
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Descript XII, 460 p. 84 illus., 59 illus. in color. online resource


The sustained skin research efforts over the past decades has led to the accumulation of a significant collection of information on skin structure and physiology as well as on the pathogenesis of cutaneous diseases.  In Molecular Dermatology: Methods and Protocols, leading experts in the field provide a collection of state-of-the-art reliable protocols covering a wide spectrum of techniques and experimental models, specific molecular assays and disease models, as well as overviews of diagnostic and research areas relevant to molecular dermatology.  As a volume in the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Comprehensive and authoritative, Molecular Dermatology: Methods and Protocols emphasizes the vital importance of skin research and collects the methodologies necessary to aid scientists in moving forward in this valuable field


Molecular Dermatology Comes of Age -- Molecular Diagnosis of Autoimmune Blistering Diseases -- Molecular Diagnosis of Genodermatoses -- Three-Dimensional Visualization of the Molecular Architecture of Cell-Cell Junctions In Situ by Cryo-Electron Tomography of Vitreous Sections -- 3D Visualization of Epidermal Langerhans Cells -- In Vivo Imaging of Lymph Node Lymphangiogenesis by Immuno-Positron Emission Tomography -- Optical Imaging of HPV Infection in a Murine Model -- Laser Scanning Microscopy Approach for Semiquantitation of In Vitro Dermal Particle Penetration -- Analysis of Cutaneous Somatic Mosaicism -- Global Proteome Analyses of SILAC Labeled Skin Cells -- MicroRNA Profiling During Human Keratinocyte Differentiation Using a Quantitative Real Time PCR Method -- Cell Death in the Skin: How to Study Its Quality and Quantity? -- In Vitro Pathogenicity Assay for Anti-Desmoglein Autoantibodies in Pemphigus -- Induction of Granulocyte-Dependent Dermal-Epidermal Separation by Autoantibodies Ex Vivo -- Analysis of Collective Invasion of Carcinoma Cells in a 3D Organotypic Model -- Isolation of Melanoma Tumor Initiating Cells from Surgical Tissues -- Detection, Enumeration, and Characterization of Immune Cells Infiltrating Melanoma Tumors -- Analysis of Cell Movement Between Skin and Other Anatomical Sites In Vivo Using Photoconvertible Fluorescent Protein “Kaede”-Transgenic Mice -- Reconstitution of Skin Fibrosis Development Using a Tissue Engineering Approach -- Bioengineered Skin Humanized Model of Psoriasis -- Induction of Contact Hypersensitivity in the Mouse Model -- Generation of Functional Multipotent Keratinocytes from Mouse Induced Pluripotent Stem Cells -- RNAi-Mediated Gene Function Analysis in Skin -- Mouse Models of Autoimmune Blistering Diseases Induced by the Passive Transfer of Antibodies -- Induction of Experimental Epidermolysis Bullosa Acquisita by Immunization with Murine Collagen VII -- Animal Model for Cutaneous Leishmaniasis -- Model for Generation of Large Numbers of Primary, Inflammatory Skin-Derived Neutrophils and Macrophages -- Zebrafish as a Model System to Study Heritable Skin Diseases -- Cell- and Protein-Based Therapy Approaches for Epidermolysis Bullosa -- RNA Trans-Splicing for Genodermatoses

Medicine Molecular biology Endocrinology Biomedicine Molecular Medicine Endocrinology


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