Title | Antiviral Resistance in Plants [electronic resource] : Methods and Protocols / edited by John M. Watson, Ming-Bo Wang |
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Imprint | Totowa, NJ : Humana Press : Imprint: Humana Press, 2012 |
Connect to | http://dx.doi.org/10.1007/978-1-61779-882-5 |
Descript | XI, 380 p. 59 illus., 10 illus. in color. online resource |
An Historical Overview of RNAi in Plants -- RNA Silencing and Antiviral Defense in Plants -- Biological Chemistry of Virus-encoded Suppressors of RNA Silencing: An Overview -- Virus-Induced Gene Silencing Using Begomovirus Satellite Molecules -- Strategies for Viral Cross Protection in Plants -- Tobacco Rattle Virus (TRV)-Based Virus-Induced Gene Silencing -- Virus-Induced Gene Silencing in Hexaploid Wheat Using Barley Stripe Mosaic Virus Vectors -- Characterization of Plant Virus-Encoded Gene Silencing Suppressors -- Generation of Plant Small RNA cDNA Libraries for High-Throughput Sequencing -- Modelling Antiviral Resistance in Plants -- Isolation and Detection of Small RNAs from Plant Tissues -- Isolation and Analysis of Small RNAs from Virus-Infected Plants -- Use of Hairpin RNA Constructs for Engineering Plant Virus Resistance -- Artificial MicroRNAs for Plant Virus Resistance -- Isolation and Biochemical Analysis of Plant Small RNAs -- Isolation and Analysis of Plant RNA-Dependent RNA Polymerases -- Plant Viroids: Isolation, Characterization/Detection, and Analysis -- Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing -- Virus-Induced Gene Silencing in Soybean -- Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in ‘Dark Green’ Islands -- Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants -- The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing -- Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication -- Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections