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TitleNanopore-Based Technology [electronic resource]
Author edited by Maria E. Gracheva
ImprintTotowa, NJ : Humana Press, 2012
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Descript XI, 267p. 85 illus., 47 illus. in color. online resource


Nanopores are vital biological features, described as tiny holes in cellular membranes used for recognition and transport of ions and molecules between compartments within the cell, as well as between the extracellular environment and the cell itself. Their study, ever growing in esteem, leads toward the promise of ultra-fast sequencing of DNA molecules with the ultimate goal of building a nanoscale device that will make rapid and cheap DNA sequencing a reality.  In Nanopore-Based Technology, expert researchers in the forefront of the field explore the cutting-edge of nanopore technology for single molecule sensing, detection, and characterization.  Divided into four convenient parts, this volume covers single molecule characterization techniques utilizing biological pores, methods for biomolecule characterization with nanoporous artificial membranes, computational studies of the biomolecule confined within the nanopore environment, as well as techniques that use novel materials in conjunction with nanopore sensing.  Written for the highly successful Methods in Molecular Biology™ series, this work provides the kind of detailed description and implementation advice that is crucial for achieving optimal results.   Authoritative and state-of-the-art, Nanopore-Based Technology serves as an excellent representation of the present-day available techniques for biomolecule characterization with nanoporous membranes in order to guide researchers toward developing the next generation of technologies for fast and cheap DNA sequencing with practically no limitations on the read lengths


Detecting and Characterizing Individual Molecules with Single Nanopores -- Protein Sensing with Engineered Protein Nanopores -- Measurements of DNA Immobilized in the Alpha-Hemolsyin Nanopore -- DNA Unzipping and Protein Unfolding Using Nanopores -- DNA Characterization with Ion Beam Sculpted Silicon Nitride Nanopores -- DNA Sequencing by Nanopore-Induced Photon Emission (SNIPE) -- Optical Tweezers for Mechanical Control Over DNA in a Nanopore -- Analyzing Single DNA Molecules by Nanopore Translocation -- DNA Characterization by Transverse Electrical Current in a Nanochannel -- Optimization of the Molecular Dynamics Method for Simulations of DNA and Ion Transport through Biological Nanopores -- Polymer Translocation Through an Electrically Tunable Nanopore in a Multilayered Semiconductor Membrane -- Graphene Nanopore Devices for DNA Sensing -- Measuring Single-Wall Carbon Nanotubes with Solid-State Nanopores -- Passive and Electrically Actuated Solid-State Nanopores for Sensing and Manipulating DNA

Chemistry Biotechnology Nanotechnology Chemistry Biotechnology Nanotechnology


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