Title | Single-Cell Analysis [electronic resource] : Methods and Protocols / edited by Sara Lindström, Helene Andersson-Svahn |
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Imprint | Totowa, NJ : Humana Press, 2012 |
Connect to | http://dx.doi.org/10.1007/978-1-61779-567-1 |
Descript | X, 238 p. 63 illus., 16 illus. in color. online resource |
Introduction: Why Analyze Single Cells? -- Flow Cytometry and Microscopy as Means of Studying Single Cells: A Short Introductional Overview -- Identification of Enzyme-Converted Peptide Products from Single Cells Using Capillary Electrophoresis and Liquid Chromatography-Mass Spectrometry -- Laser Capture Microdissection for Gene Expression Analysis of Specific Cell Populations in Single Blastocysts -- Single-Cell Culture in Microwells -- Iso-Dielectric Separation and Analysis of Cells -- Single Cell Electroporation Using Microfluidic Devices -- Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Build-In Pillar Array -- Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in the Single-Cell Comet Assay -- Droplet Microfluidics for Single-Cell Analysis -- Screening of Antigen-Specific Antibody Secreting Cells -- Analysis of Single Eukaryotic Cells Using Raman Tweezers -- Single-Cell Microinjection Technologies -- Ultrasonic Manipulation of Single Cells -- Expanding the Horizons for Single-Cell Applications on Lab-On-a-Chip Devices -- Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses