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TitlePlanar Cell Polarity [electronic resource] : Methods and Protocols
Author edited by Kursad Turksen
ImprintNew York, NY : Springer New York, 2012
Connect tohttp://dx.doi.org/10.1007/978-1-61779-510-7
Descript XI, 262 p. 59 illus., 27 illus. in color. online resource

SUMMARY

Planar cell polarity (PCP), or the alignment of a collection of cells within a cell sheet, has proven, over time, to be vital in not only normal development but also in disease states.  In Planar Cell Polarity: Methods and Protocols, expert researchers in the field present a number of detailed and well-designed protocols used successfully in their labs around the world.  As a volume in the highly successful Methods in Molecular Biology™ series, the chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Timely and authoritative, Planar Cell Polarity: Methods and Protocols serves as a valuable reference for both novices and experts in this dynamic and versatile area of study


CONTENT

Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and Dynamics at Subcellular Locations -- Analyzing Frizzled Signaling Using Fixed and Live Imaging of the Asymmetric Cell Division of the Drosophila Sensory Organ Precursor Cell -- Protein-Protein Interaction Techniques: Dissect PCP Signaling in Xenopus -- Cuticle Refraction Microscopy (CRM): A Rapid and Simple Method for Imaging Drosophila Wing Topography, an Alternative Readout of Wing Planar Cell Polarity -- Analysis of Cell Shape and Polarity during Zebrafish Gastrulation -- Analyzing Planar Cell Polarity during Zebrafish Gastrulation -- Wnt/Planar Cell Polarity Signaling in the Regulation of Convergent Extension Movements during Xenopus Gastrulation -- Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling -- Gene Loss-of-Function and Live Imaging in Chick Embryos -- Activation and Function of Small GTPases Rho, Rac, and Cdc42 during Gastrulation -- Convergent Extension Analysis in Mouse Whole Embryo Culture -- Analysis of PCP Defects in Mammalian Eye Lens -- Examining Planar Cell Polarity (PCP) in the Mammalian Cochlea -- Role of Prickle1 and Prickle2 in Neurite Outgrowth in Murine Neuroblastoma Cells -- The Planar Cell Polarity Pathway and Parietal Endoderm Cell Migration -- Analysis of Wnt/Planar Cell Polarity Pathway in Cultured Cells -- Regulation of Focal Adhesion Dynamics by Wnt5a Signaling -- The Embryonic Mouse Gut Tube as a Model for Analysis of Epithelial Polarity -- Assessing PCP in the Cochlea of Mammalian Ciliopathy Models -- Morphometric Analysis of Centrosome Position in Tissues


Life sciences Cell biology Cell physiology Life Sciences Cell Biology Cell Physiology



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