Title | Proteoglycans [electronic resource] : Methods and Protocols / edited by Françoise Rédini |
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Imprint | Totowa, NJ : Humana Press, 2012 |
Connect to | http://dx.doi.org/10.1007/978-1-61779-498-8 |
Descript | XIV, 359 p. 60 illus., 16 illus. in color. online resource |
Proteoglycans: Gene Cloning -- Proteoglycan: Site Mapping and Site-Directed Mutagenesis -- Mapping of the Wnt/β-Catenin/TCF Response Elements in the Human Versican Promoter -- Gene Silencing in Mouse Embryonic Stem Cells -- A Novel Strategy for a Splice-Variant Selective Gene Ablation: The Example of the Versican V0/V2 Knockout -- Detection of Neurocan in Cerebrospinal Fluid -- Glycosaminoglycan Chain Analysis and Characterization (Glycosylation/Epimerization) -- Characterization of Glycosaminoglycans by Tandem Vibrational Microspectroscopy and Multivariate Data Analysis -- Glycosaminoglycans: Oligosaccharide Analysis by Liquid Chromatography/Specific Labeling -- Brain Chondroitin/Dermatan Sulfate, from Cerebral Tissue to Fine Structure: Extraction, Preparation, and Fully Automated Chip-Electrospray Mass Spectrometric Analysis -- Use of Neutrons Reveal the Dynamics of Cell Surface Glycosaminoglycans -- Following Protein-Glycosaminoglycan (GAG) Polysaccharide Interactions with Differential Scanning Fluorimetry (DSF) -- In Vivo Scintigraphic Imaging of Proteoglycans -- Serglycin: The Master of the Mast Cell -- Analysis of Aggrecan Catabolism by Immunoblotting and Immunohistochemistry -- Heparan Sulfate Proteoglycans as Multifunctional Cell Regulators: Cell Surface Receptors -- Models for Studies of Proteoglycans in Kidney Pathophysiology -- Lumican Promotes Corneal Epithelial Wound Healing -- Shedding of Cell Membrane-Bound Proteoglycans -- Modulatory Effects of Proteoglycans on Proteinase Activities -- Proteoglycans and Osteolysis -- Proteoglycans and Cartilage Repair