Title | Rho GTPases [electronic resource] : Methods and Protocols / edited by Francisco Rivero |
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Imprint | New York, NY : Springer New York, 2012 |
Connect to | http://dx.doi.org/10.1007/978-1-61779-442-1 |
Descript | XIV, 407 p. 78 illus., 2 illus. in color. online resource |
Historical Overview of Rho GTPases -- Rho GTPases: Deciphering the Evolutionary History of a Complex Protein Family -- Biochemical Assays to Characterize Rho GTPases -- How to Analyze Bacterial Toxins Targeting Rho GTPases -- Assessing Ubiquitylation of Rho GTPases in Mammalian Cells -- Posttranslational Lipid Modification of Rho Family Small GTPases -- Analysis of the Role of RhoGDI1 and Isoprenylation in the Degradation of Rho GTPases -- A Quantitative Fluorometric Approach for Measuring the Interaction of RhoGDI with Membranes and Rho GTPases -- Rho GTPases and Cancer Cell Transendothelial Migration -- Quantitative and Robust Assay to Measure Cell-cell Contact Assembly and Maintenance -- Rho GTPase Knockout Induction in Primary Keratinocytes from Adult Mice -- Rho GTPase Techniques in Osteoclastogenesis -- Assessment of Rho GTPase Signaling During Neurite Outgrowth -- Assesment of the Role for Rho Family GTPases in NADPH Oxidase Activation -- Multiplex Imaging of Rho GTPase Activities in Living Cells -- FRET-Based Imaging of Rac and Cdc42 Activation During Fc-Receptor Mediated Phagocytosis in Macrophages -- High Throughput Flow Cytometry Bead-Based Multiplex Assay for Identification of Rho GTPase Inhibitors -- Functional Analysis of RhoGTPase Activation and Inhibition in a Bead-Based Miniaturized Format -- Use of Phage Display For the Identification of Molecular Sensors Specific for Activated Rho -- Identification of New Interacting Partners for Atypical Rho-GTPases: a SILAC-Based Approach -- Using Zebrafish for Studying Rho GTPases Signaling in Vivo -- Analysis of Rho GTPase Function in Axon Pathfinding Using Caenorhabditis elegans -- Protocol for ex vivo Incubation of Drosophila Primary Post-Embryonic Haemocytes for Real-Time Analyses -- Analysis of Rho GTPase Activation in Saccharomyces cerevisiae -- Assaying Rho GTPase-Dependent Processes in Dictyostelium discoideum -- FRAP-Based Analysis of Rho GTPase-dependent Polar Exocytosis in Pollen Tubes -- Assaying Rho GTPase-Dependent Processes in Dictyostelium discoideum -- FRAP-Based Analysis of Rho GTPase-dependent Polar Exocytosis in Pollen Tubes