Title | Ribosome Display and Related Technologies [electronic resource] : Methods and Protocols / edited by Julie A. Douthwaite, Ronald H. Jackson |
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Imprint | New York, NY : Springer New York, 2012 |
Connect to | http://dx.doi.org/10.1007/978-1-61779-379-0 |
Descript | XIII, 424 p. 57 illus. online resource |
Ribosome Display: A Perspective -- Preparation and Testing of E.coli S30 In Vitro Transcription Translation Extracts -- Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate -- Stabilized Ribosome Display for In Vitro Selection -- Eukaryotic Ribosome Display with In Situ DNA Recovery -- mRNA Display Using Covalent Coupling of mRNA to Translated Proteins -- SNAP Display: In Vitro Protein Evolution in Microdroplets -- cDNA Display: Rapid Stabilization of mRNA Display -- Optimization of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis -- Affinity Maturation of Phage Display Antibody Populations using Ribosome Display -- Evolution of Protein Stability Using Ribosome Display -- Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries -- Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display -- Peptide Screening Using PURE Ribosome Display -- Rapid Selection of High Affinity Binders Using Ribosome Display -- mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries -- Identification of Candidate Vaccine Genes Using Ribosome Display -- Ribosome Display for the Selection of Sac7d Scaffolds -- Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers -- Update on Pure Translation Display with Unnatural Amino Acid Incorporation -- In Vitro Selection of Unnatural Cyclic Peptide Libraries Via mRNA Display -- Optimization of CAT-354, a Therapeutic Antibody Directed Against Interleukin-13, Using Ribosome Display -- Affinity Maturation and Functional Dissection of a Humanized Anti-RAGE Monoclonal Antibody by Ribosome Display