Title | Carbohydrate Microarrays [electronic resource] : Methods and Protocols / edited by Yann Chevolot |
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Imprint | Totowa, NJ : Humana Press, 2012 |
Connect to | http://dx.doi.org/10.1007/978-1-61779-373-8 |
Descript | XVI, 427 p. 137 illus., 28 illus. in color. online resource |
Recent Advances and Future Challenges in Glycan Microarray Technology -- Chemical Synthesis of Carbohydrates and Their Surface Immobilisation: A Brief Introduction -- General Consideration on Sialic Acid Chemistry -- Synthesis of Azido-Functionalized Carbohydrates for the Design of Glycoconjugates -- Polypyrrole-Oligosaccharide Microarray for the Measurement of Biomolecular Interactions by Surface Plasmon Resonance Imaging -- Glycosylated Self-Assembled Monolayers for Arrays and Surface Analysis -- Carbohydrate Microarrays for Enzymatic Reactions and Quantification of Binding Affinities for Glycan-Protein Interactions -- Neoglycolipid (NGL)-Based Oligosaccharide Microarray System: Preparation of NGLs and Their Non-Covalent Immobilization on Nitrocellulose-Coated Glass Slides for Microarray Analyses -- Preparation of a Mannose-6-phosphate Glycan Microarray Through Fluorescent Derivatization, Phosphorylation, and Immobilization of Natural High-mannose N-glycans and its Application in Ligand Identification of P-type Lectins -- Production of Fluorous-Based Microarrays with Uncharged Carbohydrates -- General Procedure for the Synthesis of Neoglycoproteins and Immobilization on Epoxide-Modified Glass Slides -- Immobilization of Polyacrylamide-Based Glycoconjugates on Solid Phase in Immunosorbent Assays -- Surface Plasmon Resonance Imaging Analysis of Protein Binding to a Sialoside-Based Carbohydrate Microarray -- Glycoarray by DNA Directed Immobilization -- Fabrication of Carbohydrate Surfaces by Using Non Derivatised Oligosaccharides -- Polysaccharide Microarrays. Application to the Identification of Heparan Sulfate Mimetics -- Carbohydrate Antigen Microarrays -- Probing Virus-Glycan Interactions Using Glycan Microarrays -- MALDI-ToF MS Analysis of Glycosyltransferase Activities on Gold Surface Arrays -- Microarray Technology using Glycans Extracted from Natural Sources for Serum Antibody Fluorescent Detection -- Studying Modification of Aminoglycoside Antibiotics by Resistance-causing Enzymes via Microarray -- Microarray Method for the Rapid Detection of Glycosaminoglycan-Protein Interactions -- Neoglycolipid-based ‘Designer’ Oligosaccharide Microarrays to Define b-glucan Ligands for Dectin-1 -- Measurement of Antibodies to Pneumococcal Polysaccharides with Luminex xMAP Microsphere-based Liquid Arrays -- Carbohydrate Microarrays in 96-well Polystyrene Microtiter Plates -- Photoimmobilization of Saccharides -- Microwave Assisted Method for Fabrication of Carbohydrate Cluster Microarrays on 3-Dimensional Hydrazide-Dendrimer Substrate -- Combinatorial Glycoarray