Title | Plant Kinases [electronic resource] : Methods and Protocols / edited by Nico Dissmeyer, Arp Schnittger |
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Imprint | Totowa, NJ : Humana Press, 2011 |
Connect to | http://dx.doi.org/10.1007/978-1-61779-264-9 |
Descript | X, 285 p. 39 illus. online resource |
Guide to the Book Plant Kinases -- The Age of Protein Kinases -- Expression and Purification of Active Protein Kinases from Wheat Germ Extracts -- Measurement of Plant Cyclin-Dependent Kinase Activity Using Immunoprecipitation-Coupled and Affinity Purification-Based Kinase Assays and the Baculovirus Expression System -- Mitogen-Activated Protein Kinase Activity and Reporter Gene Assays in Plants -- Use of Phospho-site Substitutions to Analyze the Biological Relevance of Phosphorylation Events in Regulatory Networks -- Bacterial Assay to Study Plant Sensor Histidine Kinases.- Substrate Analysis of Arabidopsis PP2C-type Protein Phosphatase -- Modulating and Monitoring MAPK Activity During Programmed Cell Death in Pollen -- Sensitizing Plant Protein Kinases to Specific Inhibition by ATP-Competitive Molecules -- Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization -- Quantifying Degradation Rates of Trans-Membrane Receptor Kinases -- Fluorescence Correlation Spectroscopy and Fluorescence Recovery after Photobleaching to Study Receptor Kinase Mobility in planta -- Bimolecular-Fluorescence Complementation Assay to Monitor Kinase-Substrate Interactions in vivo -- Chemical Genetic Analysis of Protein Kinase Function in Plants -- Modified Metal Oxide Affinity Enrichment Combined with 2D-PAGE and Analysis of Phosphoproteomes -- Phosphoproteomics Using iTRAQ