Title | Microtubule Dynamics [electronic resource] : Methods and Protocols / edited by Anne Straube |
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Imprint | Totowa, NJ : Humana Press : Imprint: Humana Press, 2011 |
Connect to | http://dx.doi.org/10.1007/978-1-61779-252-6 |
Descript | XI, 319 p. 56 illus., 13 illus. in color. online resource |
How to Measure Microtubule Dynamics? -- Purification of Tubulin from Porcine Brain -- Purification of Tubulin from the Fission Yeast Schizosaccharomyces pombe -- Generation of Differentially Polyglutamylated Microtubules -- Preparation of Pure Tyrosinated or Detyrosinated Tubulin Isoforms -- ab-Tubulin and Microtubule Binding Assays -- Analysis of Microtubule Assembly Kinetics Using Turbidimetry -- Isolation of Centrosomes from Cultured Cells -- Preparation of Dual-Color Polarity-Marked Fluorescent Microtubule Seeds -- Reconstitution and Quantification of Dynamic Microtubule End Tracking In Vitro Using TIRF Microscopy -- Force Generation by Dynamic Microtubules In Vitro -- Variations on the Single Molecule Assay for Microtubule-Associated Proteins and Kinesins -- Analyzing the ATP Hydrolysis Cycle of Microtubule Motors -- Cryo-Electron Tomography of Microtubules Assembled In Vitro from Purified Components -- Kinetochore Microtubules, Analyzed by Correlated Light and Immuno-Electron Microscopy -- Photoactivatable-GFP-a-Tubulin as a Tool to Study Microtubule Plus-End Turnover in Living Human Cells -- Quantification of Asymmetric Microtubule Nucleation at Sub-Cellular Structures -- Investigating Microtubule Dynamic Instability Using Microtubule Targeting Agents -- L Isolation, Identification, and Validation of Microtubule-Associated Proteins from Drosophila Embryos -- Isolation of Novel +TIPs and Their Binding Partners Using Affinity Purification Techniques