Title | Integrin and Cell Adhesion Molecules [electronic resource] : Methods and Protocols / edited by Motomu Shimaoka |
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Imprint | Totowa, NJ : Humana Press : Imprint: Humana Press, 2012 |
Edition | 1 |
Connect to | http://dx.doi.org/10.1007/978-1-61779-166-6 |
Descript | XVI, 529 p. 97 illus., 10 illus. in color. online resource |
Overview: Assays for Studying Integrin-Dependent Cell Adhesion -- Cell Adhesion Assays -- Real Time Analysis of Integrin-dependent Transendothelial Migration and Integrin-independent Interstitial Motility of Leukocytes -- Lentiviral Gene Transfer Method to Study Integrin Function in T Lymphocytes -- Surface Plasmon Resonance Biosensing in Studies of the Binding between b2 Integrin I Domains and their Ligands -- Cell-Free Ligand-Binding Assays for Integrin LFA-1 -- Overview: Structural Biology of Integrins -- Protein Expression and Purification of Integrin I Domains and IgSF Ligands for Crystallography -- Electron Microscopic Imaging of Integrin -- An NMR Method to Study Protein-Protein Interactions -- Single Molecule Methods to Study Cell Adhesion Molecules -- Overview: Imaging in the Study of Integrins -- Live Imaging of LFA-1-dependent T Cell Motility and Stop Signals -- Monitoring Integrin Activation by Fluorescence Resonance Energy Transfer -- High-resolution Fluorescence Microscopy to Study Transendothelial Migration -- Multiphoton Intravital Microscopy to Study Lymphocyte Motility in Lymph Nodes -- Overview of Integrin Signaling in the Immune System -- Rap1 and Integrin Inside-out Signaling -- Isolation of Focal Adhesion Proteins for Biochemical and Proteomic Analysis -- Talin and Signaling Through Integrins -- Proteomics Method for Identification of Pseudopodium Phosphotyrosine Proteins -- Overview: Studying Integrins In Vivo -- A Method for the Generation of Conditional Gene-Targeted Mice -- T Cell Homing to the Gut Mucosa: General Concepts and Methodological Considerations -- In Vivo Quantitative Proteomics: the SILAC-Mouse -- Analysis of Chemotaxis in Dictyostelium -- Integrins in Tumor Angiogenesis & Lymphangiogenesis -- PET-Radioimmunodetection of Integrins: Imaging Acute Colitis Using a 64Cu-Labeled Anti-b7 Integrin Antibody -- Integrin-targeted Nanoparticles for siRNA Delivery.- Humanized Mice for Studying Human Leukocyte Integrins In Vivo