Title | Immune Receptors [electronic resource] : Methods and Protocols / edited by Jonathan P. Rast, James W.D. Booth |
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Imprint | Totowa, NJ : Humana Press : Imprint: Humana Press, 2011 |
Connect to | http://dx.doi.org/10.1007/978-1-61779-139-0 |
Descript | XI, 301 p. 64 illus. online resource |
Screening for Ligands of C-Type Lectin-Like Receptors -- Yeast Surface Display of Lamprey Variable Lymphocyte Receptors (VLR) -- Identification of Scavenger Receptor Ligands -- Construction, Expression, and Purification of Chimeric Protein Reagents Based on Immunoglobulin Fc Regions -- Innate Immune Receptors for Nucleic Acids -- Analysis of Receptor-Ligand Interactions by Surface Plasmon Resonance -- Cell-Based Reporter Assay to Analyze Activation of Nod1 and Nod2 -- Determining FcεRI Diffusional Dynamics Via Single Quantum Dot Tracking -- Ratiometric Analysis of Subcellular Recruitment of Fc Receptors During Phagocytosis -- Assessment of the Recycling of the Membrane-Bound Chemokine, CX3CL1 -- Measuring Immune Receptor Mobility by Fluorescence Recovery after Photobleaching -- Probing the Plasma Membrane Structure of Immune Cells through the Analysis of Membrane Sheets by Electron Microscopy -- Rapamycin-Based Inducible Translocation Systems for Studying Phagocytosis -- Micropatterned Ligand Arrays to Study Spatial Regulation in Fc Receptor Signaling -- CELLISA: Reporter Cell-Based Immunization and Screening of Hybridomas Specific for Cell Surface Antigens -- Use of Colloidal Silica-Beads for the Isolation of Cell-Surface Proteins for Mass Spectrometry-Based Proteomics -- Transfection-Based Genomic Readout for Identifying Rare Transcriptional Splice Variants -- Characterizing Somatic Hypermutation and Gene Conversion in the Chicken DT40 Cell System -- Characterizing Immune Receptors from New Genome Sequences