Title | Cystic Fibrosis [electronic resource] : Diagnosis and Protocols, Volume II: Methods and Resources to Understand Cystic Fibrosis / edited by Margarida D. Amaral, Karl Kunzelmann |
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Imprint | Totowa, NJ : Humana Press, 2011 |
Connect to | http://dx.doi.org/10.1007/978-1-61779-120-8 |
Descript | XIV, 384 p. online resource |
Introduction to Part I: Overview of Approaches to Study Cystic Fibrosis Pathophysiology -- Imaging CFTR Protein Localization in Cultured Cells and Tissues -- CFTR Regulation of Epithelial Sodium Channel -- Methods for Evaluating Inflammation in Cystic Fibrosis -- Methods for ASL Measurements and Mucus Transport Rates in Cell Cultures -- Measurement of Fluid Secretion from Intact Airway Submucosal Glands -- Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibrosis Human Airway Epithelia -- Identification and Quantification of Mucin Expression -- Methods to Classify Bacterial Pathogens in Cystic Fibrosis -- Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells -- Introduction to Part II: Omics in the Biology of Cystic Fibrosis -- Microarray mRNA Expression Profiling to Study Cystic Fibrosis -- Quantitative Differential Proteomics of Cystic Fibrosis Cell Models by SILAC (Stable Isotope Labelling in Cell Culture) -- Application of Mass Spectrometry to Study Proteomics and Interactomics in Cystic Fibrosis -- Functional Genomics Assays to Study CFTR Traffic and ENaC Function -- New Lipidomic Approaches in Cystic Fibrosis -- Introduction to Part III: Resources for CFTR Research -- Primary Epithelial Cell Models for Cystic Fibrosis Research -- Comparative Biology of Cystic Fibrosis Animal Models -- CFTR Folding Consortium: Methods Available for Studies of CFTR Folding and Correction -- Evaluation of the Disease Liability of CFTR Variants