Title | Cell Culture Techniques [electronic resource] / edited by Michael Aschner, Cristina Suñol, Anna Bal-Price |
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Imprint | Totowa, NJ : Humana Press, 2011 |
Edition | 1 |
Connect to | http://dx.doi.org/10.1007/978-1-61779-077-5 |
Descript | XIV, 498 p. 84 illus. online resource |
Guidance on Good Cell Culture Practice (GCCP) -- Induced Pluripotent Stem Cells (iPSCs): An Emerging Model System for the Study of Human Neurotoxicology -- Neural Stem Cells -- Primary Cultures for Neurotoxicity Testing -- Preparation and Use of Serum-Free Aggregating Brain Cell Cultures for Routine Neurotoxicity Screening -- Cell Culture to Investigate Neurotoxicity and Neurodegeneration Utilizing Caenorhabditis elegans -- Modeling the Blood-Brain Barrier -- In vitro Models of the Blood-Cerebrospinal Fluid Barrier and Their Use in Neurotoxicological Research -- Introducing Cloned Genes into Cultured Neurons Providing Novel In vitro Models for Neuropathology and Neurotoxicity Studies -- P19 Embryonic Carcinoma Cell Line: A Model To Study Gene-Environment Interactions -- Signal Transduction and Neurotoxicity: What Can We Learn from Experimental Culture Systems? -- Neurite Degeneration in Human Neuronal SH-SY5Y Cells as an Indicator of Axonopathy -- The Use of Differentiating N2a and C6 Cell Lines for Studies of Organophosphate Toxicity -- Assessing Toxic Injuries of Experimental Therapeutics to the Crystalline Lens Using Lens Explant Culture -- Necrosis, Apoptosis, and Autophagy: Mechanisms of Neuronal and Glial Cell Death -- Inflammation and Reactive Oxygen/Nitrogen Species in Glial/Neuronal Cultures -- Neuronal Oxidative Injury and Biomarkers of Lipid Peroxidation -- Analysis of Protein Targets by Oxidative Stress Using the Oxyblot and Biotin-Avidin-Capture Methodology -- Catecholaminergic Cell Lines for the Study of Dopamine Metabolism and Neurotoxicity -- 13C NMR Spectroscopy and Mass Spectrometry Analysis of Intermediary Metabolism in Cultured Neural Cells -- Culture Models for the Study of Amino Acid Transport and Metabolism -- Neurotransmitter Transporters and Anticonvulsant Drug Development -- Ion Channel Electrophysiology in Cultured Neurons -- Neurotoxicity Assessment by Recording Electrical Activity from Neuronal Networks on Microelectrode Array Neurochips -- GABAA Receptor Binding and Ion Channel Function in Primary Neuronal Cultures for Neuropharmacology/Neurotoxicity Testing