Title	Cell Culture Techniques [electronic resource]
Author	edited by Michael Aschner, Cristina Suñol, Anna Bal-Price
Imprint	Totowa, NJ : Humana Press, 2011
Edition	1
Connect to	http://dx.doi.org/10.1007/978-1-61779-077-5
Descript	XIV, 498 p. 84 illus. online resource

Societal, ethical, and cost-related issues, not to mention the need for sound scientific methods, have led to new and refined methods for the evaluation of health risks associated with neurotoxic compounds, relevant and predictive of exposure, relatively inexpensive, and ideally amenable to high throughput analysis and a reduction in animal use.  Cell Culture Techniques presents thorough traditional chapters, such as those on various cell culture methods that have evolved over the years, as well as innovative approaches to neurotoxicologic testing.  Accordingly, this detailed volume describes how stem cells, computational biology, and other novel powerful methods can now be applied to address the challenges of neurotoxic testing.  As part of the Neuromethods series, this work provides the kind of intensive description and implementation advice that is crucial for getting optimal results in the laboratory.   Practical and authoritative, Cell Culture Techniques serves both the novice and the experienced neurotoxicologist by inspiring the further development of mechanistically-driven, cost-effective, high throughput series of tests needed to meet the many contemporary challenges

Guidance on Good Cell Culture Practice (GCCP) -- Induced Pluripotent Stem Cells (iPSCs): An Emerging Model System for the Study of Human Neurotoxicology -- Neural Stem Cells -- Primary Cultures for Neurotoxicity Testing -- Preparation and Use of Serum-Free Aggregating Brain Cell Cultures for Routine Neurotoxicity Screening -- Cell Culture to Investigate Neurotoxicity and Neurodegeneration Utilizing Caenorhabditis elegans -- Modeling the Blood-Brain Barrier -- In vitro Models of the Blood-Cerebrospinal Fluid Barrier and Their Use in Neurotoxicological Research -- Introducing Cloned Genes into Cultured Neurons Providing Novel In vitro Models for Neuropathology and Neurotoxicity Studies -- P19 Embryonic Carcinoma Cell Line: A Model To Study Gene-Environment Interactions -- Signal Transduction and Neurotoxicity: What Can We Learn from Experimental Culture Systems? -- Neurite Degeneration in Human Neuronal SH-SY5Y Cells as an Indicator of Axonopathy -- The Use of Differentiating N2a and C6 Cell Lines for Studies of Organophosphate Toxicity -- Assessing Toxic Injuries of Experimental Therapeutics to the Crystalline Lens Using Lens Explant Culture -- Necrosis, Apoptosis, and Autophagy: Mechanisms of Neuronal and Glial Cell Death -- Inflammation and Reactive Oxygen/Nitrogen Species in Glial/Neuronal Cultures -- Neuronal Oxidative Injury and Biomarkers of Lipid Peroxidation -- Analysis of Protein Targets by Oxidative Stress Using the Oxyblot and Biotin-Avidin-Capture Methodology -- Catecholaminergic Cell Lines for the Study of Dopamine Metabolism and Neurotoxicity -- 13C NMR Spectroscopy and Mass Spectrometry Analysis of Intermediary Metabolism in Cultured Neural Cells -- Culture Models for the Study of Amino Acid Transport and Metabolism -- Neurotransmitter Transporters and Anticonvulsant Drug Development -- Ion Channel Electrophysiology in Cultured Neurons -- Neurotoxicity Assessment by Recording Electrical Activity from Neuronal Networks on Microelectrode Array Neurochips -- GABAA Receptor Binding and Ion Channel Function in Primary Neuronal Cultures for Neuropharmacology/Neurotoxicity Testing