Title | Argonaute Proteins [electronic resource] : Methods and Protocols / edited by Tom C. Hobman, Thomas F. Duchaine |
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Imprint | Totowa, NJ : Humana Press, 2011 |
Edition | 1 |
Connect to | http://dx.doi.org/10.1007/978-1-61779-046-1 |
Descript | XII, 344 p. online resource |
Purification of Native Argonaute Complexes from the Fission Yeast Schizosaccharomyces pombe -- Chromatin Immuno-Precipitation in Fission Yeast -- Biochemical Analyses of Endogenous Argonaute Complexes Immunopurified with Anti-Argonaute Monoclonal Antibodies -- Mapping of Ago2-GW182 Functional Interactions -- Continuous Density Gradients to Study Argonaute and GW182 Complexes Associated with the Endocytic Pathway -- In vitro RISC Cleavage Assay -- Native Gel Analysis for RISC Assembly -- Purification and Assembly of Human Argonaute, Dicer, and TRBP Complexes -- Detection of Human Dicer and Argonaute 2 Catalytic Activity -- Imaging the Cellular Dynamics of Drosophila Argonaute Proteins -- Live Cell Imaging of Argonaute Proteins in Mammalian Cells -- Reporter-Based Assays for Analyzing RNA Interference in Mammalian Cells -- Artificial Tethering of Argonaute Proteins for Studying their Role in Translational Repression of Target mRNAs -- An Efficient System For Let-7 MicroRNA- And GW182 Protein-Mediated Deadenylation In vitro -- Cell-Free microRNA-Mediated Translation Repression in C. elegans -- Argonaute Pull-Down and RISC Analysis Using 2'-O-Methylated Oligonucleotides Affinity Matrices -- Cloning Argonaute-Associated Small RNAs from C. elegans -- Immunoprecipitation of piRNPs and Directional, Next Generation Sequencing of piRNAs -- Generation of an Inducible Mouse ES Cell Line Deficient for Argonaute Proteins -- Whole Cell Proteome Regulation by microRNAs Captured in a Pulsed SILAC Mass Spectrometry Approach