Title | Plant Immunity [electronic resource] : Methods and Protocols / edited by John M. McDowell |
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Imprint | Totowa, NJ : Humana Press, 2011 |
Edition | 1 |
Connect to | http://dx.doi.org/10.1007/978-1-61737-998-7 |
Descript | XV, 280 p. online resource |
Studying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression -- Fragment Complementation and Co-Immunoprecipitation Assays for Understanding R Protein Structure and Function -- Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag -- Biochemical Purification of Native Immune Protein Complexes -- Chromatin Immunoprecipitation (ChIP) to Identify Global Targets of WRKY Transcription Factor Family Members Involved in Plant Immunity -- Dose Response to and Systemic Movement of Dexamethasone in the GVG Inducible Transgene System in Arabidopsis -- Quantifying Alternatively Spliced mRNA via Capillary Electrophoresis -- Constructing Haustorium-Specific cDNA Libraries from Rust Fungi -- Microaspiration of Esophageal Gland Cells and cDNA Library Construction for Identifying Parasitism Genes of Plant-Parasitic Nematodes -- Construction of Pseudomonas syringae pv. tomato DC3000 Mutant and Polymutant Strains -- A Straightforward Protocol for Electro-Transformation of Phytophthora capsici Zoospores -- Propagation, Storage, and Assays with Hyaloperonospora arabidopsidis, a Model Oomycete Pathogen of Arabidopsis -- Assaying Effector Function In planta Using Double-Barreled Particle Bombardment -- Assays for Effector-Mediated Suppression of Programmed Cell Death in Yeast -- Purification of Effector-Target Protein Complexes via Transient Expression in N. benthamiana -- Imaging Fluorescently-Tagged Phytophthora Effector Proteins Inside Infected Plant Tissue -- Immunolocalization of Pathogen Effectors -- Laser Capture Microdissection of Nematode Feeding Cells -- Laser Microdissection of Plant-Fungus Interaction Sites and Isolation of RNA for Downstream Expression Profiling -- Global Expression Profiling of RNA from Laser Microdissected Cells at Fungal-Plant Interaction Sites -- Visualizing Cellular Dynamics in Plant-Microbe Interactions Using Fluorescent-Tagged Proteins