X, 209 p. 46 illus., 10 illus. in color. online resource
SUMMARY
As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins
CONTENT
Cell-Based Microarrays: Overview -- Cell-Based Co-Transfection Microarrays for Use with HEK293T Cells on a Poly D-Lysine Polystyrene Coated Microplate -- Large-Scale Cell-Based Microarrays and Their Use with HEK293T Cells and Downstream Apoptotic Assays -- A Novel Fluorescent Transcriptional Reporter for Cell-Based Microarray Assays -- High-Throughput Subcellular Protein Localization Using Transfected-Cell Arrays -- Cell Arrays for the Measurement of Organelle Dynamics in Living Cells -- High-Throughput Immunofluorescence Microscopy Using Yeast Spheroplast Cell-Based Microarrays -- Cell-Based Microarrays of Infectious Adenovirus Encoding Short Hairpin RNA (shRNA) -- Reverse Transfected Cell Microarrays in Infectious Disease Research -- Transfected Cell Microarrays for the Expression of Membrane-Displayed Single-Chain Antibodies -- Blood Cell Capture on Antibody Micro-Arrays and Monitoring of the Cell Capture Using Surface Plasmon Resonance Imaging -- Immobilized Culture and Transfection Microarray of Nonadherent Cells -- Plasma Polymer and PEG-Based Coatings for DNA, Protein, and Cell Microarrays -- Polymer Microarrays for Cellular High-Content Screening -- High-Throughput Analyses of Gene Functions on a Cell Chip by Electroporation -- Microfluidic Image Cytometry
