In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications. In Enzyme Stabilization and Immobilization: Methods and Protocols, expert researchers explore the latest developments through detailed laboratory protocols, which address many different theories and techniques in enzyme stabilization. Chapters outline protocols for enzyme stabilization in solutions, including: liposome formation, micelle introduction, crosslinking, and additives. Secondly, the book contain protocols for enzyme stabilization via enzyme immobilization, such as sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Enzyme Stabilization and Immobilization: Methods and Protocols is an essential handbook for all molecular biologists, biochemists, and biomedical and biochemical engineers
CONTENT
Introduction to the field of enzyme immobilization and stabilization -- Stabilization of enzymes through encapsulation in liposomes -- Micellar enzymology for thermal, pH, and solvent stability -- Lipase activation and stabilization in room temperature ionic liquids -- Nanoporous silica glass for the immobilization of interactive enzyme systems -- Enzyme stabilization and immobilization by sol-gel entrapment -- Nanoporous Gold for Enzyme Immobilization -- Enzyme stabilization via bio-templated silicification reactions -- The immobilization of enzyme on Eupergit supports by covalent attachment -- Micellar polymer encapsulation of enzymes -- Enzyme Stabilization via Cross-Linked Enzyme Aggregates -- Enzyme immobilization in polyelectrolyte microcapsules -- Macroporous poly(GMA-co-EGDMA) for enzyme stabilization -- Enzyme-nanoparticle conjugates for biomedical applications -- Enzyme nanoparticle fabrication- Magnetic nanoparticle synthesis and enzyme immobilization -- Cytochrome C stabilization and immobilization in aerogels -- Kinetic Measurement for Enzyme Immobilization
