Title | Immunoelectron Microscopy [electronic resource] : Methods and Protocols / edited by Steven D. Schwartzbach, Tetsuaki Osafune |
---|---|
Imprint | Totowa, NJ : Humana Press : Imprint: Humana Press, 2010 |
Connect to | http://dx.doi.org/10.1007/978-1-60761-783-9 |
Descript | XI, 352 p. online resource |
Molecular Toolbox -- Protein Antigen Expression in Escherichia coli for Antibody Production -- Expression of Epitope-Tagged Proteins in Plants -- Expression of Epitope-Tagged Proteins in Arabidopsis Leaf Mesophyll Protoplasts -- Transient Expression of Epitope-Tagged Proteins in Mammalian Cells -- Production and Purification of Polyclonal Antibodies -- Production and Purification of Monoclonal Antibodies -- Production of Antipeptide Antibodies -- Preparation of Colloidal Gold Particles and Conjugation to Protein A, IgG, F(ab’)2, and Streptavidin -- Microscopy Toolbox -- Immunoelectron Microscopy of Chemically Fixed Developing Plant Embryos -- Pre-embedding Immunogold Localization of Antigens in Mammalian Brain Slices -- Pre-embedding Immunoelectron Microscopy of Chemically Fixed Mammalian Tissue Culture Cells -- Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues -- In Vivo Cryotechniques for Preparation of Animal Tissues for Immunoelectron Microscopy -- Immunoelectron Microscopy of Cryofixed Freeze-Substituted Mammalian Tissue Culture Cells -- Immunoelectron Microscopy of Cryofixed Freeze-Substituted Saccharomyces cerevisiae -- High-Resolution Molecular Localization by Freeze-Fracture Replica Labeling -- Pre-embedding Electron Microscopy Methods for Glycan Localization in Chemically Fixed Mammalian Tissue Using Horseradish Peroxidase-Conjugated Lectin -- Pre-embedding Nanogold Silver and Gold Intensification -- The Post-embedding Method for Immunoelectron Microscopy of Mammalian Tissues: A Standardized Procedure Based on Heat-Induced Antigen Retrieval -- Double-Label Immunoelectron Microscopy for Studying the Colocalization of Proteins in Cultured Cells -- Serial Section Immunoelectron Microscopy of Algal Cells -- Freeze-Etch Electron Tomography for the Plasma Membrane Interface -- Localization of rDNA at Nucleolar Structural Components by Immunoelectron Microscopy -- Immunogold Labelling for Scanning Electron Microscopy -- Horseradish Peroxidase as a Reporter Gene and as a Cell-Organelle-Specific Marker in Correlative Light-Electron Microscopy -- Monitoring Rapid Endocytosis in the Electron Microscope via Photoconversion of Vesicles Fluorescently Labeled with FM1-43