Title | Protein Misfolding and Cellular Stress in Disease and Aging [electronic resource] : Concepts and Protocols / edited by Peter Bross, Niels Gregersen |
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Imprint | Totowa, NJ : Humana Press : Imprint: Humana Press, 2010 |
Connect to | http://dx.doi.org/10.1007/978-1-60761-756-3 |
Descript | XI, 330 p. 54 illus., 4 illus. in color. online resource |
Concepts -- Protein Misfolding and Cellular Stress: An Overview -- Protein Aggregation Diseases: Toxicity of Soluble Prefibrillar Aggregates and Their Clinical Significance -- Consequences of Stress in the Secretary Pathway: The ER Stress Response and Its Role in the Metabolic Syndrome -- What Role Does Mitochondrial Stress Play in Neurodegenerative Diseases? -- Autophagy in Disease -- Mitophagy and Mitoptosis in Disease Processes -- Cellular Stress and Protein Misfolding During Aging -- Measuring Consequences of Protein Misfolding and Cellular Stress Using OMICS Techniques -- Protocols -- Production of Cells with Targeted Integration of Gene Variants of Human ABC Transporter for Stable and Regulated Expression Using the Flp Recombinase System -- Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress -- Examining Ubiquitinated Protein Aggregates in Tissue Sections -- Determination of Proteasomal Activities -- Measurement of Autophagy in Cells and Tissues -- Transcription Factor Sequestration by Polyglutamine Proteins -- Biological Membranes as Protein Aggregation Matrices and Targets of Amyloid Toxicity -- Measurement of Mitochondrial ROS Production -- In Vivo Detection of Oxidized Proteins: A Practical Approach to Tissue-Derived Mitochondria -- Measurement of Oxidized/Reduced Glutathione Ratio -- Determination of Altered Mitochondria Ultrastructure by Electron Microscopy -- Assessing Bad Sub-cellular Localization Under Conditions Associated with Prevention or Promotion of Mitochondrial Permeability Transition-Dependent Toxicity -- A Cellular Viability Assay to Monitor Drug Toxicity -- Rescue of Misfolded Proteins and Stabilization by Small Molecules