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TitleLiposomes [electronic resource] : Methods and Protocols, Volume 2: Biological Membrane Models
Author edited by Volkmar Weissig
ImprintTotowa, NJ : Humana Press, 2010
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Descript XVI, 548 p. 126 illus., 7 illus. in color. online resource


With nearly one hundred years of intensive study, lipids have proven to be a vital and ever-more-promising area of cell biological research. In Liposomes: Methods and Protocols, leading experts in the related fields explore cutting-edge experimental methods involving all aspects of lipids as essential components of the cell membrane. Volume 2: Biological Membrane Models focuses on detailed methods for the use of liposomes in studying a variety of biochemical and biophysical membrane phenomena concomitant with chapters describing a great palette of state-of-the-art analytical technologies. As a volume in the highly successful Methods in Molecular Biology™ series, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Liposomes: Methods and Protocols promises to be an essential source of practical know-how for every investigator, young and seasoned alike, whose research area involves in one way or another phospholipids, glycolipids, or cholesterol


Utilization of Liposomes for Studying Drug Transfer and Uptake -- The Use of Liposomes in the Study of Drug Metabolism: A Method to Incorporate the Enzymes of the Cytochrome P450 Monooxygenase System into Phospholipid, Bilayer Vesicles -- Use of Liposomes to Study Cellular Osmosensors -- Studying Mechanosensitive Ion Channels Using Liposomes -- Studying Amino Acid Transport Using Liposomes -- Use of Liposomes for Studying Interactions of Soluble Proteins with Cellular Membranes -- Liposomal Reconstitution of Monotopic Integral Membrane Proteins -- The Reconstitution of Actin Polymerization on Liposomes -- Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains under Physiological Ionic-Strength Conditions -- Visualization of Lipid Domain-Specific Protein Sorting in Giant Unilamellar Vesicles -- Biosynthesis of Proteins Inside Liposomes -- Study of Respiratory Cytochromes in Liposomes -- Use of Liposomes to Evaluate the Role of Membrane Interactions on Antioxidant Activity -- Studying Colloidal Aggregation Using Liposomes -- Assessment of Liposome–Cell Interactions -- Methods to Monitor Liposome Fusion, Permeability, and Interaction with Cells -- The Use of Isothermal Titration Calorimetry to Study Multidrug Transport Proteins in Liposomes -- Studying Lipid Organization in Biological Membranes Using Liposomes and EPR Spin Labeling -- Membrane Translocation Assayed by Fluorescence Spectroscopy -- Interaction of Lipids and Ligands with Nicotinic Acetylcholine Receptor Vesicles Assessed by Electron Paramagnetic Resonance Spectroscopy -- Environmental Scanning Electron Microscope Imaging of Vesicle Systems -- Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution Scale -- Atomic Force Microscopy for the Characterization of Proteoliposomes -- Method of Simultaneous Analysis of Liposome Components Using HPTLC/FID -- Viscometric Analysis of DNA-Lipid Complexes -- Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes -- Fluorescence Resonance Energy Transfer-Based Analysis of Lipoplexes -- Analysis of Lipoplex Structure and Lipid Phase Changes -- Fluorescence Methods for Evaluating Lipoplex-Mediated Gene Delivery -- FRET Imaging of Cells Transfected with siRNA/Liposome Complexes -- Spectral Bio-Imaging and Confocal Imaging of the Intracellular Distribution of Lipoplexes -- Techniques for Loading Technetium-99m and Rhenium-186/188 Radionuclides into Pre-formed Liposomes for Diagnostic Imaging and Radionuclide Therapy -- Fluorescence Correlation Spectroscopy for the Study of Membrane Dynamics and Organization in Giant Unilamellar Vesicles -- Liposome Biodistribution via Europium Complexes -- Biosensor-Based Evaluation of Liposomal Binding Behavior -- Use of Liposomes to Study Vesicular Transport

Medicine Pharmacology Lipids Cell biology Cell membranes Biomedicine Pharmacology/Toxicology Cell Biology Membrane Biology Lipidology


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