Title | Live Cell Imaging [electronic resource] : Methods and Protocols / edited by Dmitri B. Papkovsky |
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Imprint | Totowa, NJ : Humana Press, 2010 |
Edition | 1 |
Connect to | http://dx.doi.org/10.1007/978-1-60761-404-3 |
Descript | XI, 367 p. 101 illus., 8 illus. in color. online resource |
I: General Principles and Overview -- Instrumentation for Live-Cell Imaging and Main Formats -- Labels and Probes for Live Cell Imaging: Overview and Selection Guide -- Live Cell Imaging: An Industrial Perspective -- II: Imaging Techniques, Probes, and Applications -- Design of Fluorescent Fusion Protein Probes -- Synthetic Fluorescent Probes for Imaging of Peroxynitrite and Hypochlorous Acid in Living Cells -- Photo-Activatable Probes for the Analysis of Receptor Function in Living Cells -- The Application of Fluorescent Probes for the Analysis of Lipid Dynamics During Phagocytosis -- Imaging of Mitotic Cell Division and Apoptotic Intra-Nuclear Processes in Multicolor -- Manipulation of Neutrophil-Like HL-60 Cells for the Study of Directed Cell Migration -- A Method for Analyzing Protein–Protein Interactions in the Plasma Membrane of Live B Cells by Fluorescence Resonance Energy Transfer Imaging as Acquired by Total Internal Reflection Fluorescence Microscopy -- Sample Preparation for STED Microscopy -- Two-Photon Permeabilization and Calcium Measurements in Cellular Organelles -- Imaging and Analysis of Three-Dimensional Cell Culture Models -- Long-Term Imaging in Microfluidic Devices -- Monitoring of Cellular Responses to Hypoxia -- Imaging of Cellular Oxygen and Analysis of Metabolic Responses of Mammalian Cells -- Analysis of Mitochondrial pH and Ion Concentrations -- Live Cell Imaging Analysis of Receptor Function -- Subcellular Dynamic Imaging of Protein–Protein Interactions in Live Cells by Bioluminescence Resonance Energy Transfer -- Quantitative Analysis of Membrane Potentials -- Image Correlation Spectroscopy to Define Membrane Dynamics