Title | Cytoskeleton Methods and Protocols [electronic resource] / edited by Ray H. Gavin |
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Imprint | Totowa, NJ : Humana Press, 2010 |
Connect to | http://dx.doi.org/10.1007/978-1-60761-376-3 |
Descript | XII, 391 p. 77 illus. online resource |
Live-Cell Imaging of the Cytoskeleton -- High Resolution Multimode Light Microscopy of Cell Migration: Long-Term Imaging and Analysis -- Imaging the Cytoskeleton in Live Xenopus laevis Embryos -- Live-Cell Imaging of the Cytoskeleton and Mitochondrial–Cytoskeletal Interactions in Budding Yeast -- Dynamics of Actin Filaments and Microtubules -- Jasplakinolide: An Actin-Specific Reagent that Promotes Actin Polymerization -- Analysis of Centrosome Function and Microtubule Dynamics by Time-Lapse Microscopy in Xenopus Egg Extracts -- Fluorescence Microscopy Protocols for Investigating Cytoskeleton Components -- Quantitative Fluorescence Microscopy Techniques -- A FRET-Based Approach for Studying Conformational Changes of a Cytoskeleton-Related Tumor Suppressor Molecule -- Sample Preparation for Fluorescence Imaging of the Cytoskeleton in Fixed and Living Plant Roots -- Imaging of the Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells -- Electron Microscopy Protocols for Imaging the Cytoskeleton -- Imaging Cytoskeleton Components by Electron Microscopy -- Purification and Localization of Intraflagellar Transport Particles and Polypeptides -- High Pressure Freezing, Electron Microscopy, and Immuno-Electron Microscopy of Tetrahymena thermophila Basal Bodies -- Strategies for Imaging Microtubules in Plant Cells -- Analysis of Cell and Organelle Motility -- A Rapid Tracking Method for the Quantitative Analysis of Organelle Streaming Velocity -- Melanosome Motility in Fish Retinal Pigment Epithelial Cells -- Analysis of Properties of Cilia Using Tetrahymena thermophila -- Application of Cell Traction Force Microscopy for Cell Biology Research -- 2D and 3D Quantitative Analysis of Cell Motility and Cytoskeletal Dynamics -- Detergent-Extracted Models for the Study of Cilia or Flagella -- Genetic and Proteomics Approaches for Cytoskeleton Protein Function -- The Analysis of Intermediate Filament Dynamics Using Transfections and Cell Fusions -- Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila -- Proteomic Tools for the Analysis of Cytoskeleton Proteins