Title | Heterologous Expression of Membrane Proteins [electronic resource] : Methods and Protocols / edited by Isabelle Mus-Veteau |
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Imprint | Totowa, NJ : Humana Press, 2010 |
Connect to | http://dx.doi.org/10.1007/978-1-60761-344-2 |
Descript | XII, 272 p. 44 illus., 5 illus. in color. online resource |
Heterologous Expression of Membrane Proteins for Structural Analysis -- Production of Membrane Proteins in Escherichia coli and Lactococcus lactis -- Mammalian Membrane Receptors Expression as Inclusion Bodies in Escherichia coli -- Expression of Membrane Proteins at the Escherichia coli Membrane for Structural Studies -- Membrane Protein Expression in Lactococcus lactis -- Heterologous Expression of Human Membrane Receptors in the Yeast Saccharomyces cerevisiae -- Mammalian Membrane Protein Expression in Baculovirus-Infected Insect Cells -- Expression of Membrane Proteins in Drosophila Melanogaster S2 Cells: Production and Analysis of a EGFP-Fused G Protein-Coupled Receptor as a Model -- Membrane Protein Expression in the Eyes of Transgenic Flies -- Expression of Mammalian Membrane Proteins in Mammalian Cells Using Semliki Forest Virus Vectors -- Membrane Protein Expression in Cell-Free Systems -- Practical Considerations of Membrane Protein Instability during Purification and Crystallisation -- Membrane Protein Solubilization -- Amphipols and Fluorinated Surfactants: Two Alternatives to Detergents for Studying Membrane Proteins In vitro -- Heterologous Expression and Affinity Purification of Eukaryotic Membrane Proteins in View of Functional and Structural Studies: The Example of the Sarcoplasmic Reticulum Ca2+-ATPase